肝素与尼罗罗非鱼皮肤胶原交联递送骨形态发生蛋白-2促进大鼠颅骨骨缺损修复。

IF 4.4 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Lina Ma, Li Fu, Chengxu Gu, Haonan Wang, Zhenghai Yu, Xiuwei Gao, Dongmei Zhao, Baosheng Ge, Naili Zhang
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引用次数: 0

摘要

胶原蛋白是一种广泛应用于组织再生的生物材料。目前,源自鱼类的水胶原蛋白由于其热稳定性不足,在生物医学材料应用方面的探索很少。为了提高鱼类胶原蛋白的骨修复能力和热稳定性,我们将罗非鱼皮肤胶原蛋白用EDC/NHS与肝素交联,特异性结合BMP-2。采用差示扫描量热法(DSC)测定了罗非鱼皮交联肝素胶原蛋白(HC-COL)的热稳定性。CCK-8法检测MC3T3-E1细胞增殖,评价HC-COL的细胞毒性。通过体外诱导MC3T3-E1细胞分化,检测BMP-2与HC-COL的特异性结合,并评价BMP-2负载HC-COL (HC-COL-BMP-2)的生物活性。在体内,通过显微ct和组织学观察评估HC-COL-2的骨修复能力。EDC/NHS交联后,尼罗罗非鱼胶原的肝素联性和热稳定性同时得到改善。HC-COL没有细胞毒性。此外,BMP-2与HC-COL的结合明显增加。此外,体外实验还揭示了BMP-2结合HC-COL的有效生物活性,可诱导MC3T3-E1细胞具有较高的ALP活性并形成矿化结节。体内研究表明,HC-COL-BMP-2组实现了更多的矿化和成熟骨形成。制备的HC-COL是一种有效的BMP-2结合载体,具有足够的热稳定性,可作为一种有用的骨修复生物材料。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Delivery of bone morphogenetic protein-2 by crosslinking heparin to nile tilapia skin collagen for promotion of rat calvaria bone defect repair.

Collagen has been widely used as a biomaterial for tissue regeneration. At the present, aqua-collagen derived from fish is poorly explored for biomedical material applications due to its insufficient thermal stability. To improve the bone repair ability and thermal stability of fish collagen, the tilapia skin collagen was crosslinked by EDC/NHS with heparin to bind specifically to BMP-2. The thermal stability of tilapia skin collagen crosslinked with heparin (HC-COL) was detected by differential scanning calorimetry (DSC). Cytotoxicity of HC-COL was assessed by detecting MC3T3-E1 cell proliferation using CCK-8 assay. The specific binding of BMP-2 to HC-COL was tested and the bioactivity of BMP-2-loaded HC-COL (HC-COL-BMP-2) was evaluated in vitro by inducing MC3T3-E1 cell differentiation. In vivo, the bone repair ability of HC-COL-2 was evaluated using micro-CT and histological observation. After crosslinking by EDC/NHS, the heparin-linked and the thermostability of the collagen of Nile Tilapia were improved simultaneously. HC-COL has no cytotoxicity. In addition, the binding of BMP-2 to HC-COL was significantly increased. Furthermore, the in vitro study revealed the effective bioactivity of BMP-2 binding on HC-COL by inducing MC3T3-E1 cells with higher ALP activity and the formation of mineralized nodules. In vivo studies showed that more mineralized and mature bone formation was achieved in HC-COL-BMP-2 group. The prepared HC-COL was an effective BMP-2 binding carrier with enough thermal stability and could be a useful biomaterial for bone repair.

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来源期刊
Progress in Biomaterials
Progress in Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
9.60
自引率
4.10%
发文量
35
期刊介绍: Progress in Biomaterials is a multidisciplinary, English-language publication of original contributions and reviews concerning studies of the preparation, performance and evaluation of biomaterials; the chemical, physical, biological and mechanical behavior of materials both in vitro and in vivo in areas such as tissue engineering and regenerative medicine, drug delivery and implants where biomaterials play a significant role. Including all areas of: design; preparation; performance and evaluation of nano- and biomaterials in tissue engineering; drug delivery systems; regenerative medicine; implantable medical devices; interaction of cells/stem cells on biomaterials and related applications.
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