Circ_0006220通过miR-342-3p/GOT2轴促进NSCLC进展。

IF 1.1 4区 医学 Q4 CARDIAC & CARDIOVASCULAR SYSTEMS
Jichun Tang, Xuan Li, Lili Zhao, Jiajun Hui, Ning Ding
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引用次数: 1

摘要

目的:失调的环状rna (circRNAs)在非小细胞肺癌(NSCLC)的肿瘤发生中显示出至关重要的调节功能。本研究旨在探讨circ_0006220在非小细胞肺癌中的生物学功能及调控理论。方法:采用逆转录-定量聚合酶链反应法和Western blot法分别检测RNA和蛋白的表达。共收集73例NSCLC肿瘤样本进行表达分析,并采用A-549和NCI-H1299细胞系进行功能实验。通过细胞计数试剂盒-8法、集落形成法、5-乙基-2′-脱氧尿苷法和流式细胞术评估细胞增殖情况。通过流式细胞术、transwell实验和毛细血管样网络形成实验分析细胞凋亡、运动和血管生成能力。采用双荧光素酶报告基因法和RNA免疫沉淀法验证靶关系。结果:Circ_0006220在NSCLC组织和细胞系中高表达。Circ_0006220沉默抑制非小细胞肺癌细胞的增殖、迁移、侵袭和血管生成,但诱导细胞凋亡。Circ_0006220作为microRNA-342-3p (miR-342-3p)海绵,Circ_0006220敲低诱导的NSCLC细胞表型变化在很大程度上被miR-342-3p敲低所推翻。miR-342-3p与谷草转氨酶2 (GOT2)的3'非翻译区相互作用,GOT2过表达在NSCLC细胞中大大降低了miR-342-3p过表达介导的影响。Circ_0006220可通过海绵转染miR-342-3p上调GOT2的表达。结论:Circ_0006220通过介导miR-342-3p/GOT2调控级联促进NSCLC细胞的恶性行为。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Circ_0006220 Contributes to NSCLC Progression through miR-342-3p/GOT2 Axis.

Circ_0006220 Contributes to NSCLC Progression through miR-342-3p/GOT2 Axis.

Circ_0006220 Contributes to NSCLC Progression through miR-342-3p/GOT2 Axis.

Circ_0006220 Contributes to NSCLC Progression through miR-342-3p/GOT2 Axis.

Purpose: Dysregulated circular RNAs (circRNAs) have shown crucial modulatory functions in tumorigenesis, containing non-small cell lung cancer (NSCLC). The purpose of this study was to explore the biological functions and regulatory theory of circ_0006220 in NSCLC.

Methods: Reverse transcription-quantitative polymerase chain reaction and Western blot assay were conducted to measure RNA and protein expression, respectively. A total of 73 cases of NSCLC tumor samples were collected for expression analysis, and A-549 and NCI-H1299 cell lines were used for functional experiments. Cell proliferation was assessed by cell counting kit-8 assay, colony formation assay, 5-ethynyl-2'-deoxyuridine assay, and flow cytometry. Cell apoptosis, motility, and angiogenesis ability were analyzed by flow cytometry, transwell assays, and capillary-like network formation assay. Dual-luciferase reporter assay and RNA immunoprecipitation assay were conducted to verify the target relationships.

Results: Circ_0006220 was highly expressed in NSCLC tissues and cell lines. Circ_0006220 silencing inhibited the proliferation, migration, invasion, and angiogenesis but induced the apoptosis of NSCLC cells. Circ_0006220 acted as a microRNA-342-3p (miR-342-3p) sponge, and circ_0006220 knockdown-induced changes on the phenotypes of NSCLC cells were largely overturned by the knockdown of miR-342-3p. miR-342-3p interacted with the 3' untranslated region of glutamic-oxaloacetic transaminase 2 (GOT2), and GOT2 overexpression largely diminished miR-342-3p overexpression-mediated influences in NSCLC cells. Circ_0006220 could up-regulate GOT2 expression by sponging miR-342-3p.

Conclusion: Circ_0006220 promoted the malignant behaviors of NSCLC cells through mediating the miR-342-3p/GOT2 regulation cascade.

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来源期刊
Annals of Thoracic and Cardiovascular Surgery
Annals of Thoracic and Cardiovascular Surgery CARDIAC & CARDIOVASCULAR SYSTEMS-SURGERY
CiteScore
2.80
自引率
0.00%
发文量
56
审稿时长
4-8 weeks
期刊介绍: Information not localized
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