基于海藻酸钠和聚n-乙烯基己内酰胺的白蛋白负载热/pH双响应纳米凝胶。

IF 4.4 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Hamed Mohammad Gholiha, Morteza Ehsani, Ardeshir Saeidi, Azam Ghadami
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引用次数: 1

摘要

在过去的几十年里,许多研究人员试图将药物封装在生物聚合物纳米凝胶中作为注射药物。本文报道了用海藻酸钠接枝聚n -乙烯基己内酰胺(PNVCL)制备双反应性牛血清白蛋白(BSA)纳米凝胶。首先通过自由基聚合法制备pnvcl -g-海藻酸钠(PNVCL-g-Alg),然后在牛血清白蛋白存在下,将海藻酸钠离子交联制备纳米凝胶。FTIR光谱显示PNVCL-g-Alg纳米凝胶制备成功。浊度法和流变学分析表明,云点温度接近人体。通过FE-SEM和动态光散射对颗粒尺寸进行评价,发现干燥和膨胀状态下的颗粒尺寸分别约为30 nm和280 nm。考察了温度和pH对牛血清白蛋白释放的影响。通过对药物释放行为的比较,我们发现蛋白质在云点以上温度下的释放速度比在云点以下温度下的释放速度快。在pH为5.5和7.4时,对bsa负载的PNVCL-g-Alg的pH敏感性进行了评估,结果表明,酸性条件下药物释放速度快于中性条件。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Albumin-loaded thermo/pH dual-responsive nanogels based on sodium alginate and poly (N-vinyl caprolactam).

During the past decades, many researchers have tried to encapsulate medicines in biopolymer nanogels as injectable medicines. In the present study, dual-responsive bovine serum albumin (BSA)-loaded nanogels prepared from sodium alginate grafted poly (N-vinyl caprolactam) (PNVCL) have been reported. First, PNVCL-g-sodium alginate (PNVCL-g-Alg) was synthesized through free radical polymerization, and then nanogels were obtained from ionic crosslinking of sodium alginate in the presence of BSA. FTIR spectra showed that PNVCL-g-Alg nanogels were successfully prepared. Turbidimetry and rheometry analyses demonstrated the cloud point temperature near the human body. Particle size was evaluated using FE-SEM and dynamic light scattering and it was found that the size of particles in dry and swollen state are about 30 and 280 nm, respectively. The effect of temperature and pH on BSA release was evaluated. By comparing the drug release behavior, we found that the release of the protein at the temperature above the cloud point is faster than that at the temperature below the cloud point. The pH sensitivity of BSA-loaded PNVCL-g-Alg was evaluated at pH 5.5 and 7.4 and showed that the drug release was faster at acidic pH than at neutral pH.

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来源期刊
Progress in Biomaterials
Progress in Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
9.60
自引率
4.10%
发文量
35
期刊介绍: Progress in Biomaterials is a multidisciplinary, English-language publication of original contributions and reviews concerning studies of the preparation, performance and evaluation of biomaterials; the chemical, physical, biological and mechanical behavior of materials both in vitro and in vivo in areas such as tissue engineering and regenerative medicine, drug delivery and implants where biomaterials play a significant role. Including all areas of: design; preparation; performance and evaluation of nano- and biomaterials in tissue engineering; drug delivery systems; regenerative medicine; implantable medical devices; interaction of cells/stem cells on biomaterials and related applications.
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