{"title":"一种改进的水凝胶生产方案，以减少细胞含量。","authors":"Gabriela Catão Diniz Braga, Cristina Pires Camargo, Martin Conrad Harmsen, Aristides Tadeu Correia, Sonia Souza, Marilia Seelaender, Viviane Araujo Nunes, Jeniffer Farias Dos Santos, Elida Adalgisa Neri, Iuri Cordeiro Valadão, Luiz Felipe Pinho Moreira, Rolf Gemperli","doi":"10.1590/acb371005","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>To analyze the cytotoxicity and cell in porcine-derived decellularized skin matrix.</p><p><strong>Methods: </strong>We analyzed the effect of multiple decellularization processes by histological analysis, DNA quantification, and flow cytometry. Subsequently, we analyzed the most appropriate hydrogel concentration to minimize cytotoxicity on fibroblast culture and to maximize cell proliferation.</p><p><strong>Results: </strong>After the fourth decellularization, the DNA quantification showed the lowest DNA concentration (< 50 ng/mg). Histological analysis showed no cell components in the hydrogel. Moreover, hematoxylin and eosin showed a heterogeneous structure of collagen fibers. The best hydrogel concentration ranged from 3 to 25%, and there was no significant difference between the 24 hours and seven days.</p><p><strong>Conclusions: </strong>The process of hydrogel production was effective for removing cells and DNA elements. The best hydrogel concentration ranged from 3 to 25%.</p>","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"37 10","pages":"e371005"},"PeriodicalIF":1.1000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9762429/pdf/","citationCount":"0","resultStr":"{\"title\":\"A modified hydrogel production protocol to decrease cellular content.\",\"authors\":\"Gabriela Catão Diniz Braga, Cristina Pires Camargo, Martin Conrad Harmsen, Aristides Tadeu Correia, Sonia Souza, Marilia Seelaender, Viviane Araujo Nunes, Jeniffer Farias Dos Santos, Elida Adalgisa Neri, Iuri Cordeiro Valadão, Luiz Felipe Pinho Moreira, Rolf Gemperli\",\"doi\":\"10.1590/acb371005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>To analyze the cytotoxicity and cell in porcine-derived decellularized skin matrix.</p><p><strong>Methods: </strong>We analyzed the effect of multiple decellularization processes by histological analysis, DNA quantification, and flow cytometry. Subsequently, we analyzed the most appropriate hydrogel concentration to minimize cytotoxicity on fibroblast culture and to maximize cell proliferation.</p><p><strong>Results: </strong>After the fourth decellularization, the DNA quantification showed the lowest DNA concentration (< 50 ng/mg). Histological analysis showed no cell components in the hydrogel. Moreover, hematoxylin and eosin showed a heterogeneous structure of collagen fibers. The best hydrogel concentration ranged from 3 to 25%, and there was no significant difference between the 24 hours and seven days.</p><p><strong>Conclusions: </strong>The process of hydrogel production was effective for removing cells and DNA elements. The best hydrogel concentration ranged from 3 to 25%.</p>\",\"PeriodicalId\":6992,\"journal\":{\"name\":\"Acta cirurgica brasileira\",\"volume\":\"37 10\",\"pages\":\"e371005\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9762429/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta cirurgica brasileira\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1590/acb371005\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"SURGERY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta cirurgica brasileira","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1590/acb371005","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"SURGERY","Score":null,"Total":0}
A modified hydrogel production protocol to decrease cellular content.
Purpose: To analyze the cytotoxicity and cell in porcine-derived decellularized skin matrix.
Methods: We analyzed the effect of multiple decellularization processes by histological analysis, DNA quantification, and flow cytometry. Subsequently, we analyzed the most appropriate hydrogel concentration to minimize cytotoxicity on fibroblast culture and to maximize cell proliferation.
Results: After the fourth decellularization, the DNA quantification showed the lowest DNA concentration (< 50 ng/mg). Histological analysis showed no cell components in the hydrogel. Moreover, hematoxylin and eosin showed a heterogeneous structure of collagen fibers. The best hydrogel concentration ranged from 3 to 25%, and there was no significant difference between the 24 hours and seven days.
Conclusions: The process of hydrogel production was effective for removing cells and DNA elements. The best hydrogel concentration ranged from 3 to 25%.
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