重组人绒毛膜促性腺激素刺激后液相色谱-串联质谱(LC-MS/MS)测定睾酮的建议截断点。

IF 2.4 4区医学 Q2 DEVELOPMENTAL BIOLOGY

Sexual Development Pub Date : 2022-01-01 DOI:10.1159/000519422

Leticia R de Oliveira, Carlos A Longui, Guilherme Guaragna-Filho, José L da Costa, Rafael Lanaro, Maria I Chiamolera, Maricilda P de Mello, André M Morcillo, Andrea T Maciel-Guerra, Gil Guerra-Junior

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引用次数: 2

摘要

评估性腺甾体生成的人绒毛膜促性腺激素(hCG)刺激试验在评估46,xy性发育障碍(DSD)患者中至关重要。本研究旨在通过LC-MS/MS确定单剂量重组人绒毛膜促性腺激素(rhCG)刺激后睾丸激素(T)的截止水平，该截止水平表明睾丸功能正常。对19例46、XY、DSD、T分泌正常的青春期前儿童进行了评价。采用液相色谱-串联质谱(LC-MS/MS)技术测定250µg rhCG应用前和应用后7 d的T和双氢睾酮(DHT)水平。我们建议用LC-MS/MS分析rhCG刺激后T的临界值为0.89 ng/mL。

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Suggested Cutoff Point for Testosterone by Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS) after Stimulation with Recombinant Human Chorionic Gonadotropin.

The human chorionic gonadotropin (hCG) stimulation test that evaluates gonadal steroidogenesis is crucial in the assessment of patients with 46,XY disorders of sex development (DSD). This study aimed to determine a testosterone (T) cutoff level that indicates an adequate testicular function using LC-MS/MS after stimulation with recombinant human chorionic gonadotropin (rhCG) in a single dose. Nineteen prepubertal children with 46,XY DSD and normal T secretion were evaluated. T and dihydrotestosterone (DHT) levels were measured by liquid chromatography technique with tandem mass spectrometry (LC-MS/MS) before and 7 days after rhCG application at 250 µg. We suggest 0.89 ng/mL as the cutoff point for T after rhCG stimulation analyzed by LC-MS/MS.

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来源期刊

Sexual Development 生物-发育生物学

CiteScore

4.00

自引率

4.30%

发文量

审稿时长

>12 weeks

期刊介绍： Recent discoveries in experimental and clinical research have led to impressive advances in our knowledge of the genetic and environmental mechanisms governing sex determination and differentiation, their evolution as well as the mutations or endocrine and metabolic abnormalities that interfere with normal gonadal development. ‘Sexual Development’ provides a unique forum for this rapidly expanding field. Its broad scope covers all aspects of genetics, molecular biology, embryology, endocrinology, evolution and pathology of sex determination and differentiation in humans and animals. It publishes high-quality original research manuscripts, review articles, short reports, case reports and commentaries. An internationally renowned and multidisciplinary editorial team of three chief editors, ten prominent scientists serving as section editors, and a distinguished panel of editorial board members ensures fast and author-friendly editorial processing and peer reviewing.