杉木素对HTR-8/SVneo人滋养细胞氧化损伤的细胞保护和基因保护作用。

IF 2.5 4区 医学 Q3 GENETICS & HEREDITY
Mutagenesis Pub Date : 2023-02-03 DOI:10.1093/mutage/geac013
Marija Bruić, Andrea Pirković, Aleksandra Vilotić, Milica Jovanović-Krivokuća, Biljana Spremo-Potparević
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引用次数: 2

摘要

胎盘中活性氧的增加和氧化失衡已被认为是导致妊娠并发症的重要因素。在怀孕期间摄入富含抗氧化剂的食物可以起到保护作用,预防不良后果,如先兆子痫、流产等。在大量的动物研究中,以及在体外对各种人类细胞类型的研究中,黄酮类taxifolin已经显示出许多促进健康的作用。然而,其对人类胎盘细胞——滋养细胞的影响尚未确定。因此,本研究探讨了杉木素对氧化应激诱导的滋养细胞HTR-8/SVneo的细胞保护和基因保护作用。使用MTT和结晶紫测定法评估了杉木素浓度范围(1-150µM)的细胞毒性。通过将HTR-8/SVneo细胞暴露于H2O2,建立了氧化应激模型。为了确定细胞保护和抗原毒性作用,将细胞与三种浓度的紫杉醇(10、50和100µM)预孵育,然后暴露于H2O2中。结晶紫法检测,浓度为1、5、10、25、50和100µM的紫杉醇素对HTR-8/SVneo细胞无细胞毒作用,但浓度为150µM的紫杉醇素可显著减少贴壁细胞数量。MTT实验表明,选定浓度的紫杉醇预处理对h2o2诱导的细胞毒性有显著的细胞保护作用。此外,通过彗星测定,taxifolin显示h2o2诱导的DNA损伤显著减少。本研究显示了紫杉醇素对氧化损伤的人滋养细胞的保护作用。需要进一步的研究来探索潜在的机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytoprotective and genoprotective effects of taxifolin against oxidative damage in HTR-8/SVneo human trophoblast cells.

An increase of reactive oxygen species in the placenta and oxidative disbalance has been recognized as a significant factor contributing to pregnancy complications. Dietary intake of food rich in antioxidants during pregnancy could exert a protective role in the prevention of adverse outcomes such as preeclampsia, miscarriage, and others. Flavonoid taxifolin has shown numerous health-promoting effects in a large number of studies conducted on animals, as well as various human cell types in vitro. However, its effects on human placental cells-trophoblasts-have yet to be determined. Therefore, cytoprotective and genoprotective effects of taxifolin on trophoblast cell line HTR-8/SVneo under induced oxidative stress were explored in this study. Cytotoxicity of a range of taxifolin concentrations (1-150 µM) was evaluated using the MTT and crystal violet assays. A model of oxidative stress was achieved by exposing HTR-8/SVneo cells to H2O2. To determine cytoprotective and antigenotoxic effects, the cells were pre-incubated with three concentrations of taxifolin (10, 50, and 100 µM) and then exposed to H2O2. Taxifolin in concentrations of 1, 5, 10, 25, 50, and 100 µM showed no cytotoxic effects on HTR-8/SVneo cells, but 150 µM of taxifolin caused a significant decrease in adherent cell number, as detected by crystal violet assay. Pretreatment with the chosen concentrations of taxifolin showed a significant cytoprotective effect on H2O2-induced cytotoxicity, as determined by the MTT assay. Furthermore, taxifolin showed a significant reduction in H2O2-induced DNA damage, measured by comet assay. This study showed protective effects of taxifolin on human trophoblast cells exposed to oxidative damage. Further studies are needed to explore the underlying mechanisms.

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来源期刊
Mutagenesis
Mutagenesis 生物-毒理学
CiteScore
5.90
自引率
3.70%
发文量
22
审稿时长
6-12 weeks
期刊介绍: Mutagenesis is an international multi-disciplinary journal designed to bring together research aimed at the identification, characterization and elucidation of the mechanisms of action of physical, chemical and biological agents capable of producing genetic change in living organisms and the study of the consequences of such changes.
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