从发酵肉中分离出的益生菌具有很高的抗氧化和抗炎潜力。

IF 2.5 4区 医学 Q3 GENETICS & HEREDITY
Mutagenesis Pub Date : 2023-02-03 DOI:10.1093/mutage/geac023
Deni Kostelac, Marko Gerić, Goran Gajski, Jadranka Frece
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引用次数: 2

摘要

益生菌的应用及其与不同食物基质的结合是影响不健康饮食如微生物群失调、炎症和氧化应激等新出现问题的方法之一。发现和选择合适的益生菌是一个具有挑战性的过程,特别是发酵肉制品,也被描述为弹性益生菌微生物的潜在来源。本研究的目的是研究从传统发酵肉制品中分离的植物乳杆菌1K益生菌的潜在有益特性。此外,提取少量益生菌代谢物,并在脂多糖刺激的人外周血单个核细胞(PBMCs)炎症模型中检测其抗炎活性。还确定了代谢物的安全特性,包括细胞毒性和遗传毒性。所研究的益生菌菌株通过活细胞和代谢产物部分表现出较高的抗氧化能力。活细胞经胃肠道转运后仍保持良好的抗氧化活性。提取的益生菌代谢物显著抑制lps刺激的PBMC中TNF-α的产生,从而发挥抗炎作用。单独的代谢物对分离的免疫细胞没有细胞毒性或基因毒性活性。所得结果表明,利用发酵肉制品作为特定益生菌来源的可能性,可能为消费者提供抗氧化和抗炎益处。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Probiotic bacteria isolated from fermented meat displays high antioxidant and anti-inflammatory potential.

One of the ways to impact emerging problems of unhealthy diet such as microbiota dysbiosis, inflammation, and oxidative stress is the application of probiotics and their incorporation into different food matrices. Discovery and selection of appropriate probiotic bacteria is challenging procedure especially for fermented meat products that have also been described as a potential source of resilient probiotic microorganisms. The aim of this study was to investigate probiotic bacteria Lactiplantibacillus plantarum 1K isolated from traditional fermented meat product for its potential beneficial properties. Furthermore, small probiotic metabolites were extracted, and their anti-inflammatory activity was tested in a lipopolysaccharide-stimulated inflammatory model on human peripheral blood mononuclear cells (PBMCs). Safety characteristics of metabolites including cytotoxicity and genotoxicity were also determined. Investigated probiotic strain exerted high antioxidant potential by viable cells but also by metabolite fraction. Viable cells retained the satisfactory antioxidant activity after gastrointestinal transit. Extracted probiotic metabolites significantly inhibited TNF-α production in LPS-stimulated PBMC thus exerting anti-inflammatory activity. Metabolites alone showed no cytotoxic or genotoxic activity toward isolated immune cells. Obtained results indicate the possibility to use fermented meat products as sources for specific probiotics that might provide antioxidant and anti-inflammatory benefits for the consumers.

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来源期刊
Mutagenesis
Mutagenesis 生物-毒理学
CiteScore
5.90
自引率
3.70%
发文量
22
审稿时长
6-12 weeks
期刊介绍: Mutagenesis is an international multi-disciplinary journal designed to bring together research aimed at the identification, characterization and elucidation of the mechanisms of action of physical, chemical and biological agents capable of producing genetic change in living organisms and the study of the consequences of such changes.
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