菊苣叶水醇提取物对吸烟大鼠睾丸芳烃受体表达的影响。

IF 1.9 Q3 CHEMISTRY, MEDICINAL
Maryam Hashemi, Mahnaz Azarnia, Zahra Hajebrahimi, Samad Nejad Ebrahimi
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引用次数: 0

摘要

目的:香烟烟雾(CS)中含有活性氧(ROS)等化合物。过量ROS引起的氧化应激最终导致生殖细胞凋亡和男性不育。菊苣的叶子富含天然抗氧化剂,但它们对CS对睾丸组织的不良影响的保护作用尚未得到研究。材料和方法:24只Wistar大鼠分为四组:对照组、提取物组:用菊苣提取物(200mg/kg体重/天)处理13周,烟雾组:暴露于CS 13周,以及烟雾+提取物组:暴露在CS并用C.intybus提取物处理。进行组织学和生化分析以及细胞凋亡测定,并测定Ahr和Cyp1a1的表达。结果:C.intybus治疗可补偿CS引起的支持细胞、精原细胞、精母细胞和精子细胞的减少。菊苣提取物减少自由基,改善抗氧化状态。在提取物组和烟雾组中分别观察到最低和最高百分比的凋亡细胞,而同时用C.intybus提取物处理导致凋亡细胞显著减少。对照组、提取物组、烟雾组和烟雾+提取物组的平均Ahr水平分别为1.00±0.57、1.93±0.25、5.98±0.42和0.62±0.22(p 0.05),和0.21±0.23(p 0.05)。结论:西洋参提取物可能通过下调Ahr而影响Cyp1a1的表达。这些导致自由基和细胞凋亡的减少,以及抗氧化状态的改善。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The effect of hydroalcoholic extract of <i>Cichorium intybus</i> leaf on aryl hydrocarbon receptor expression in the testis of Wistar rats exposed to cigarette smoke.

The effect of hydroalcoholic extract of <i>Cichorium intybus</i> leaf on aryl hydrocarbon receptor expression in the testis of Wistar rats exposed to cigarette smoke.

The effect of hydroalcoholic extract of <i>Cichorium intybus</i> leaf on aryl hydrocarbon receptor expression in the testis of Wistar rats exposed to cigarette smoke.

The effect of hydroalcoholic extract of Cichorium intybus leaf on aryl hydrocarbon receptor expression in the testis of Wistar rats exposed to cigarette smoke.

Objective: Cigarette smoke (CS) contains compounds such as reactive oxygen species (ROS). Oxidative stress caused by excessive ROS eventually leads to germ cell apoptosis and male infertility. The leaves of Cichorium intybus (chicory) are rich in natural antioxidants, but their protective effects on the adverse effects of CS on testicular tissue have not been studied.

Materials and methods: 24 Wistar rats were classified into four groups: control, extract: treatment with chicory extract (200 mg/kg body weight/day) for 13 weeks, smoke: exposed to CS for 13 weeks, and smoke + extract: exposed to CS and treated with the C. intybus extract. Histological and biochemical analyses and apoptosis assay were done, and Ahr, and Cyp1a1 expression was determined.

Results: Treatment with C. intybus compensated for the reduction of Sertoli cells, spermatogonia, spermatocytes, and spermatids caused by CS. Chicory extract reduced free radicals and improved antioxidant status. The lowest and highest percentage of apoptotic cells was observed in the extract and smoke groups, respectively, while simultaneous treatment with C. intybus extract led to a significant reduction of apoptotic cells. The mean Ahr levels in the control, extract, smoke and smoke + extract groups were 1.00±0.57, 1.93±0.25, 5.98±0.42, and 0.62±0.22, respectively (p˂0.05). The mean levels of Cyp1a1 expression in the control, extract, smoke and smoke + extract groups were 1.00±0.31, 2.28±0.65, 5.55±0.40, and 0.21±0.23 (p˂0.05).

Conclusion: The C. intybus extract probably affected Cyp1a1 expression by downregulation of Ahr. These led to a decrease in free radicals and apoptosis, and an improvement in antioxidant status.

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来源期刊
Avicenna Journal of Phytomedicine
Avicenna Journal of Phytomedicine CHEMISTRY, MEDICINAL-
CiteScore
3.40
自引率
4.50%
发文量
17
审稿时长
6 weeks
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