针刺通过RhoA/ROCK通路改善SAMP8小鼠突触可塑性

IF 1.8 4区 医学 Q3 CLINICAL NEUROLOGY
Bohong Kan, Zhengjia Dong, Zhenyu Tang, Lan Zhao, Zhen Li
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引用次数: 0

摘要

背景:研究发现突触可塑性损伤是阿尔茨海默病(AD)的早期标志。RhoA/ROCK通路参与突触可塑性的调节。针刺可显著改善AD患者的认知状态。目的:利用现代生物学技术检测SAMP8小鼠突触可塑性和RhoA/ROCK通路的变化,以及针刺的干预作用。方法:采用Morris水迷宫和电生理技术检测小鼠空间记忆和LTP的变化。采用高尔基Cox染色法和CASEVIEWER2.1软件定量分析小鼠海马树突棘形态和数量的变化。采用下拉法和ELISA法分别检测小鼠海马组织中RhoA和ROCK2的活性。WB技术检测小鼠海马组织中ROCK2蛋白表达及MLC2、LIMK2、CRMP2磷酸化水平。结果:8月龄SAMP8小鼠的神经行为和突触可塑性明显受损。针刺可提高SAMP8小鼠的空间学习记忆能力,部分阻止海马根尖树突次枝棘数减少和LTP的衰减。8月龄SAMP8小鼠海马RhoA/ROCK通路明显激活,针刺对其有抑制作用。结论:针刺可通过抑制RhoA/ROCK通路的异常激活来改善突触可塑性,提高AD的空间学习记忆能力,从而达到治疗AD的目的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Acupuncture Improves Synaptic Plasticity of SAMP8 Mice through the RhoA/ROCK Pathway.

Background: Studies have found synaptic plasticity damage to be an early marker of Alzheimer's disease (AD). RhoA/ROCK pathway is involved in the regulation of synaptic plasticity. Acupuncture can significantly improve the cognitive state of AD.

Objective: We aimed to use modern biological technology to detect the changes in synaptic plasticity and RhoA/ROCK pathway in SAMP8 mice, as well as the intervention effect of acupuncture.

Methods: Morris water maze and electrophysiological techniques were used in vivo to detect the changes in spatial memory and LTP of mice. Golgi Cox staining and CASEVIEWER2.1 software were used to quantitatively analyze the changes in the morphology and number of dendritic spines in the hippocampus of mice. The activity of RhoA and ROCK2 in the hippocampus of mice was detected, respectively, by pull-down technique and ELISA. WB technique was used to detect the protein expression of ROCK2 and phosphorylation level of MLC2, LIMK2, and CRMP2 in the hippocampus of mice.

Results: The neurobehavior and synaptic plasticity of 8-month-old SAMP8 mice were found to be significantly impaired. Acupuncture could improve the spatial learning and memory ability of SAMP8 mice, and partially prevent the reduction in the number of spines on the secondary branches of the apical dendrites in the hippocampus and the attenuation of LTP. The RhoA/ROCK pathway was significantly activated in the hippocampus of 8-month-old SAMP8 mice, and acupuncture had an inhibitory effect on it.

Conclusion: Acupuncture can improve synaptic plasticity by inhibiting the abnormal activation of the RhoA/ROCK pathway, and improve the spatial learning and memory ability of AD, so as to achieve the purpose of treating AD.

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来源期刊
Current Alzheimer research
Current Alzheimer research 医学-神经科学
CiteScore
4.00
自引率
4.80%
发文量
64
审稿时长
4-8 weeks
期刊介绍: Current Alzheimer Research publishes peer-reviewed frontier review, research, drug clinical trial studies and letter articles on all areas of Alzheimer’s disease. This multidisciplinary journal will help in understanding the neurobiology, genetics, pathogenesis, and treatment strategies of Alzheimer’s disease. The journal publishes objective reviews written by experts and leaders actively engaged in research using cellular, molecular, and animal models. The journal also covers original articles on recent research in fast emerging areas of molecular diagnostics, brain imaging, drug development and discovery, and clinical aspects of Alzheimer’s disease. Manuscripts are encouraged that relate to the synergistic mechanism of Alzheimer''s disease with other dementia and neurodegenerative disorders. Book reviews, meeting reports and letters-to-the-editor are also published. The journal is essential reading for researchers, educators and physicians with interest in age-related dementia and Alzheimer’s disease. Current Alzheimer Research provides a comprehensive ''bird''s-eye view'' of the current state of Alzheimer''s research for neuroscientists, clinicians, health science planners, granting, caregivers and families of this devastating disease.
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