TMEM200A高表达与胃癌预后不良和免疫浸润相关。

IF 2.3 4区医学 Q3 ONCOLOGY

Pathology & Oncology Research Pub Date : 2023-01-01 DOI:10.3389/pore.2023.1610893

Hongyang Deng, Tengfei Li, Fengxian Wei, Wei Han, Xiaodong Xu, Youcheng Zhang

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引用次数: 2

摘要

背景:胃癌是全球范围内发病率高、预后差的恶性肿瘤之一。探索新的GC分子标志物对改善GC预后具有重要意义。跨膜蛋白200A (TMEM200A)是跨膜蛋白(TMEM)家族的一员。本研究首次探讨了TMEM200A在GC中的潜在功能及其与免疫浸润的关系。方法:通过Cancer Genome Atlas (TCGA)和Gene expression Omnibus (GEO)数据库检测TMEM200A的差异表达。绘制受试者工作特征(ROC)曲线，评价TMEM200A对GC的诊断价值。采用Wilcoxon检验和Kruskal-Wallis检验探讨TMEM200A与胃癌患者临床特征的关系。采用Kaplan-Meier法、Log-rank检验、单因素/多因素Cox回归分析和nomogram预测模型来确定TMEM200A对总生存期(OS)的影响。利用共表达基因和基因集富集分析(GSEA)探索TMEM200A的潜在生物学功能。我们使用肿瘤免疫估计资源(Tumor Immune Estimation Resource, TIMER)数据库和ssGSEA算法来估计TMEM200A与免疫细胞浸润的关系。此外，我们使用TCGA-STAD数据集研究了TMEM200A与免疫检查点/免疫细胞表面标记物的相关性。最后，我们使用MethSurv确定了TMEM200A中与预后相关的甲基化位点。结果:TMEM200A在GC组织中高表达。TMEM200A对GC有较好的诊断价值。TMEM200A高表达可能缩短GC患者的OS，可能是GC患者OS的独立危险因素。TMEM200A参与构建预测模型，对胃癌患者1、3、5年生存率有较好的预测效果。共表达基因和GSEA提示TMEM200A可能是一种与肿瘤侵袭转移密切相关的粘附分子。此外，TMEM200A可能与免疫细胞浸润和免疫检查点表达显著相关。我们还发现TMEM200A含有三个与预后不良相关的甲基化位点。结论:TMEM200A表达上调可能是胃癌的预后标志物，且与肿瘤微环境(tumor microenvironment, TME)密切相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

High expression of TMEM200A is associated with a poor prognosis and immune infiltration in gastric cancer.

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High expression of TMEM200A is associated with a poor prognosis and immune infiltration in gastric cancer.

Background: Gastric cancer (GC) is one of the global malignant tumors with high incidence and poor prognosis. Exploring new GC molecular markers is important to improve GC prognosis. Transmembrane protein 200A (TMEM200A) is a member of the family of transmembrane proteins (TMEM). This study is the first to investigate the potential function of TMEM200A and its relationship with immune infiltration in GC. Methods: The differential expression of TMEM200A was determined through the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. The receiver operating characteristic (ROC) curve was drawn to assess the diagnostic value of TMEM200A for GC. The relationship between TMEM200A and the clinical characteristics of patients with GC was investigated using the Wilcoxon test and the Kruskal-Wallis test. The effect of TMEM200A on overall survival (OS) was identified using the Kaplan-Meier method, the Log-rank test, the univariate/multivariate Cox regression analysis, and the nomogram prediction model. The co-expressed genes and gene set enrichment analysis (GSEA) were used to explore the potential biological functions of TMEM200A. We used the Tumor Immune Estimation Resource (TIMER) database and the ssGSEA algorithm to estimate the relationship between TMEM200A and immune cell infiltration. Furthermore, we investigated the correlation of TMEM200A with immune checkpoint/immune cell surface markers using the TCGA-STAD data set. Finally, we identified prognosis-related methylation sites in TMEM200A using MethSurv. Results: TMEM200A was highly expressed in GC tissues. TMEM200A had a good diagnostic value for GC. High expression of TMEM200A may shorten the OS of GC patients and may be an independent risk factor for OS in GC patients. TMEM200A participates in the construction of a predictive model with a good predictive effect on the survival rate of GC patients at 1, 3, and 5 years. Co-expressed genes and GSEA indicated that TMEM200A may be an adhesion molecule closely associated with tumor invasion and metastasis. In addition, TMEM200A may be significantly associated with immune cell infiltration and immune checkpoint expression. We also found that TMEM200A contains three methylation sites associated with a poor prognosis. Conclusion: Upregulated TMEM200A may be a promising prognostic marker for GC and is closely associated with the tumor microenvironment (TME).

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来源期刊

Pathology & Oncology Research 医学-病理学

CiteScore

6.30

自引率

0.00%

发文量

134

审稿时长

4-8 weeks

期刊介绍： Pathology & Oncology Research (POR) is an interdisciplinary Journal at the interface of pathology and oncology including the preclinical and translational research, diagnostics and therapy. Furthermore, POR is an international forum for the rapid communication of reviews, original research, critical and topical reports with excellence and novelty. Published quarterly, POR is dedicated to keeping scientists informed of developments on the selected biomedical fields bridging the gap between basic research and clinical medicine. It is a special aim for POR to promote pathological and oncological publishing activity of colleagues in the Central and East European region. The journal will be of interest to pathologists, and a broad range of experimental and clinical oncologists, and related experts. POR is supported by an acknowledged international advisory board and the Arányi Fundation for modern pathology.