西班牙马德里某三级医院Allplex NG-DR测定淋病奈瑟菌大环内酯类和氟喹诺酮类耐药相关突变的流行情况。

IF 1.9 4区医学 Q4 MICROBIOLOGY

Revista Espanola De Quimioterapia Pub Date : 2023-12-01 Epub Date: 2023-09-18 DOI:10.37201/req/058.2023

A Maldonado-Barrueco, C Sanz-González, I Falces-Romero, P García-Clemente, J Cacho-Calvo, I Quiles-Melero

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引用次数: 0

摘要

目的:耐药淋病奈瑟菌(NG)感染呈上升趋势。研究报道，NG菌株普遍存在A2059G/C2611T (rRNA23S)和S91F (parC)突变，从而对阿奇霉素和环丙沙星产生耐药性。方法:我们进行了一项前瞻性队列研究，评估了2022年10月至2023年1月期间从马德里一家三级医院的一组患者中收集的首次空尿、直肠和口咽拭子。样品采用Allplex 7-STI Essential Assay (Seegene)筛选。采用Allplex NG-DR法(Seegene)检测耐药情况。结果:共纳入1415例患者，其中NG感染阳性样本112例。1例患者有C2611T突变(0.9%)，两例患者均未出现A2059G突变。我们发现67例(59.8%)s91f阳性患者。44例(39.3%)患者无任何突变。结论:我们报告了NG感染中大环内酯类A2059G/C2611T突变的低患病率和S91F的高患病率。分子方法检测NG耐药性可用于直接非培养样品。

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Prevalence of mutations associated with macrolide and fluoroquinolone resistance in Neisseria gonorrhoeae with Allplex NG-DR Assay (Seegene) in a tertiary hospital from Madrid, Spain.

Objective: The prevalence of drug-resistant Neisseria gonorrhoeae (NG) infections is increasing. Studies report the prevalence of NG strains presenting A2059G/C2611T (rRNA23S) and S91F (parC) mutations conferring resistance to azithromycin and ciprofloxacin.

Methods: We conducted a prospective cohort study evaluating first void-urine urines, rectal, and oropharyngeal swabs collected from a cohort of patients in a tertiary hospital in Madrid between October 2022 and January 2023. Samples were screened by Allplex 7-STI Essential Assay (Seegene). Drug resistances were performed by Allplex NG-DR Assay (Seegene).

Results: A total of 1,415 patients were included, of which 112 had a positive sample for NG infection. One patient had a C2611T mutation (0.9%) and neither patient showed A2059G mutation. We found 67 (59.8%) S91F-positive patients. Forty-four patients (39.3%) not had any mutations.

Conclusions: We report a low-prevalence of mutations A2059G/C2611T to macrolides and a high-prevalence to S91F in NG infections. Molecular methods for the detection of NG resistance could be useful in direct non-culturable samples.

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来源期刊

Revista Espanola De Quimioterapia 医学-药学

CiteScore

2.90

自引率

10.50%

发文量

146

审稿时长

>12 weeks

期刊介绍： The official journal of the Sociedad Española de Quimioterapia (Spanish Society of Chemotherapy), publishes articles that further knowledge and advance the science and application of antimicrobial chemotherapy with antibiotics and antifungal, antiviral and antiprotozoal agents primarily in human medicine. Authors sign an exclusive license agreement, where authors have copyright but license exclusive rights in their article to the Publisher. All manuscripts are free open access. Revista Española de Quimioterapia includes the following sections: reviews, original articles, brierf reports, letters, and consensus documents.