Hsa_Circ_0005044通过调节miR-296-3p/FOSL1促进牙髓干细胞成骨/成牙分化

IF 2.6 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Zhongjun Liu, Siwei Li, Shuaimei Xu, Nu Er Bi Ya A Bu Du Xi Ku, Jun Wen, Xiongqun Zeng, Xiaoqing Shen, Pingping Xu
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引用次数: 0

摘要

环状rna (circRNAs)是一种缺乏编码潜能的rna。这些环状rna在牙髓干细胞(DPSC)成骨/牙源性分化中的作用仍有待确定。在本研究中,通过RNA-seq分析了DPSC成骨/牙源性分化过程中的circRNA表达谱。采用qRT-PCR证实circ_0005044、miR-296-3p和FOSL1在DPSC成骨分化过程中的差异表达。Circ_0005044、miR-296-3p和FOSL1被敲低或过表达。通过碱性磷酸酶(ALP)和茜素红S (ARS)染色检测成骨细胞活性和相关矿物质活性。通过荧光素酶报告基因检测评估miR-296-3p、circ_0005044和FOSL1之间的相互作用。最后,利用体内系统证实circ_0005044与成骨细胞分化的相关性。结果,我们在DPSC成骨/牙源性分化过程中检测到显著的circ_0005044和FOSL1上调,以及伴随的miR-296-3p下调。当敲除circ_0005044或过表达miR-296-3p时,可显著抑制成骨。荧光素酶报告基因检测证实miR-296-3p能够结合circ_0005044和FOSL1野生型中的保守序列。此外,在体内敲除circ_0005044显著减弱骨形成。因此,circ_0005044/miR-2964-3p/FOSL1轴调控DPSC成骨/牙源性分化,可能为牙髓复合体再生提供潜在的分子靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hsa_Circ_0005044 Promotes Osteo/Odontogenic Differentiation of Dental Pulp Stem Cell Via Modulating miR-296-3p/FOSL1.

Circular RNAs (circRNAs) are a form of RNAs that lack coding potential. The role of such circRNAs in dental pulp stem cell (DPSC) osteo/odontogenic differentiation remains to be determined. In this study, circRNA expression profiles in DPSC osteo/odontogenic differentiation process were analyzed by RNA-seq. qRT-PCR was used to confirm the differential expression of circ_0005044, miR-296-3p, and FOSL1 in DPSC osteogenic differentiation process. Circ_0005044, miR-296-3p, and FOSL1 were knocked down or overexpressed. Osteoblastic activity and associated mineral activity were monitored via alkaline phosphatase (ALP) and alizarin red S (ARS) staining. Interactions between miR-296-3p, circ_0005044, and FOSL1 were assessed through luciferase reporter assays. Finally, an in vivo system was used to confirm the relevance of circ_0005044 to osteoblastic differentiation. As results, we detected significant circ_0005044 and FOSL1 upregulation in DPSC osteo/odontogenic differentiation process, as well as concomitant miR-296-3p downregulation. When knocking down circ_0005044 or overexpressed miR-296-3p, this significantly inhibited osteogenesis. Luciferase reporter assay confirmed that miR-296-3p was capable of binding to conserved sequences in the wild-type forms of both the circ_0005044 and FOSL1. Furthermore, knocking down circ_0005044 in vivo significantly attenuated bone formation. Therefore, the circ_0005044/miR-2964-3p/FOSL1 axis regulates DPSC osteo/odontogenic differentiation, which may provide potential molecular targets for dental-pulp complex regeneration.

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来源期刊
DNA and cell biology
DNA and cell biology 生物-生化与分子生物学
CiteScore
6.60
自引率
0.00%
发文量
93
审稿时长
1.5 months
期刊介绍: DNA and Cell Biology delivers authoritative, peer-reviewed research on all aspects of molecular and cellular biology, with a unique focus on combining mechanistic and clinical studies to drive the field forward. DNA and Cell Biology coverage includes: Gene Structure, Function, and Regulation Gene regulation Molecular mechanisms of cell activation Mechanisms of transcriptional, translational, or epigenetic control of gene expression Molecular Medicine Molecular pathogenesis Genetic approaches to cancer and autoimmune diseases Translational studies in cell and molecular biology Cellular Organelles Autophagy Apoptosis P bodies Peroxisosomes Protein Biosynthesis and Degradation Regulation of protein synthesis Post-translational modifications Control of degradation Cell-Autonomous Inflammation and Host Cell Response to Infection Responses to cytokines and other physiological mediators Evasive pathways of pathogens.
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