甾体受体辅激活因子TAIMAN是一种新型的昆虫生物钟调节剂。

IF 4.5 2区 生物学 Q1 Agricultural and Biological Sciences
PLoS Genetics Pub Date : 2023-09-08 eCollection Date: 2023-09-01 DOI:10.1371/journal.pgen.1010924
Vlastimil Smykal, Lenka Chodakova, Marketa Hejnikova, Kristina Briedikova, Bulah Chia-Hsiang Wu, Hana Vaneckova, Ping Chen, Anna Janovska, Pavlina Kyjakova, Martin Vacha, David Dolezel
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引用次数: 2

摘要

TAIMAN(TAI)是哺乳动物类固醇受体辅激活子(SRCs)中唯一的昆虫直系同源物,是蜕皮激素和幼激素(JH)信号通路的关键调节剂,控制昆虫的发育和繁殖。这种调节作用是由JH依赖性TAI与JH受体甲氧嘧啶耐受性的异二聚体以及与Ecdysone受体复合物的结合介导的。昆虫激素与光周期和热周期等非生物线索协同调节昆虫的生理和发育。在这里,我们通过显微外科手术、激素和激素模拟物的应用以及基因敲除的组合,测试了JH和蜕皮激素信号对linden bug Pyrrocoris apterus雄性生物钟的影响。通过三个不重叠的双链RNA片段中的每一个使taiman沉默,使自由运行期(FRP)显著减慢至27-29小时,而对照组为24小时。为了进一步证实泰曼在昆虫昼夜节律中的时钟调节功能,我们对德国小蠊进行了泰曼敲除。尽管小蠊和Pyrrhocoris谱系分离了~380 mya,但德国小蠊的沉默使FRP减慢了2个多小时,这表明(至少)在一些昆虫群体中具有保守的TAI时钟功能。有趣的是,无论是通过阻断JH和蜕皮激素的合成,还是通过应用激素或其模拟物,还是通过敲低相应的激素受体,都没有改变林登虫昼夜节律时钟的节奏。我们的研究结果促进了TAI作为一种新的生物钟调节剂的作用,这是首次在昆虫中描述的作用。我们推测,TAI在生物钟中的参与与哺乳动物SRC-2在协调代谢和昼夜节律中的作用一致,并且TAI/SRC可能是动物生物钟的保守成分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Steroid receptor coactivator TAIMAN is a new modulator of insect circadian clock.

Steroid receptor coactivator TAIMAN is a new modulator of insect circadian clock.

Steroid receptor coactivator TAIMAN is a new modulator of insect circadian clock.

Steroid receptor coactivator TAIMAN is a new modulator of insect circadian clock.

TAIMAN (TAI), the only insect ortholog of mammalian Steroid Receptor Coactivators (SRCs), is a critical modulator of ecdysone and juvenile hormone (JH) signaling pathways, which govern insect development and reproduction. The modulatory effect is mediated by JH-dependent TAI's heterodimerization with JH receptor Methoprene-tolerant and association with the Ecdysone Receptor complex. Insect hormones regulate insect physiology and development in concert with abiotic cues, such as photo- and thermoperiod. Here we tested the effects of JH and ecdysone signaling on the circadian clock by a combination of microsurgical operations, application of hormones and hormone mimics, and gene knockdowns in the linden bug Pyrrhocoris apterus males. Silencing taiman by each of three non-overlapping double-strand RNA fragments dramatically slowed the free-running period (FRP) to 27-29 hours, contrasting to 24 hours in controls. To further corroborate TAIMAN's clock modulatory function in the insect circadian clock, we performed taiman knockdown in the cockroach Blattella germanica. Although Blattella and Pyrrhocoris lineages separated ~380 mya, B. germanica taiman silencing slowed the FRP by more than 2 hours, suggesting a conserved TAI clock function in (at least) some insect groups. Interestingly, the pace of the linden bug circadian clock was neither changed by blocking JH and ecdysone synthesis, by application of the hormones or their mimics nor by the knockdown of corresponding hormone receptors. Our results promote TAI as a new circadian clock modulator, a role described for the first time in insects. We speculate that TAI participation in the clock is congruent with the mammalian SRC-2 role in orchestrating metabolism and circadian rhythms, and that TAI/SRCs might be conserved components of the circadian clock in animals.

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来源期刊
PLoS Genetics
PLoS Genetics 生物-遗传学
CiteScore
8.10
自引率
2.20%
发文量
438
审稿时长
1 months
期刊介绍: PLOS Genetics is run by an international Editorial Board, headed by the Editors-in-Chief, Greg Barsh (HudsonAlpha Institute of Biotechnology, and Stanford University School of Medicine) and Greg Copenhaver (The University of North Carolina at Chapel Hill). Articles published in PLOS Genetics are archived in PubMed Central and cited in PubMed.
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