草鱼养殖水体古卡假单胞菌ZS1的全基因组分析。

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Genome Pub Date : 2023-01-01 DOI:10.1139/gen-2022-0055
Shuhui Niu, Wangbao Gong, Zhifei Li, Kai Zhang, Guangjun Wang, Ermeng Yu, Yun Xia, Jingjing Tian, Hongyan Li, Jiajia Ni, Jun Xie
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引用次数: 1

摘要

从草鱼(Ctenopharyngodon idellus)培养水中分离出的古卡假单胞菌ZS1,表现出有效的好氧硝酸盐还原而不积累亚硝酸盐;然而,这种有氧硝酸盐还原的分子途径尚不清楚。在本研究中,我们构建了P. furukawaii ZS1的完整基因组图谱,并与参考菌株进行了比较基因组分析。结果表明,古氏疟原虫ZS1基因组大小为6 ~ 26 ~ 050 bp,包含5427个预测蛋白编码序列。基因组中含有异化硝态氮还原氨途径所需的全部基因,但缺乏同化硝态氮还原途径所需的基因;此外,还发现了将氨转化为有机氮的基因。与氮和氧化磷酸化途径相关的假定基因的存在表明,ZS1在有氧条件下可以同时进行呼吸和硝酸盐还原,从而通过反硝化作用迅速消耗亚硝酸盐进行解毒。本研究的目的是指出菌株ZS1在未来水产养殖中大规模应用的巨大潜力。这项工作在分子水平上对假单胞菌物种的氮代谢途径提供了见解。对氮代谢途径的了解也为假单胞菌的进一步改造和发育提供了重要的分子信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Complete genome analysis of Pseudomonas furukawaii ZS1 isolated from grass carp (Ctenopharyngodon idellus) culture water.

Pseudomonas furukawaii ZS1, isolated from grass carp (Ctenopharyngodon idellus) culture water, exhibits efficient aerobic nitrate reduction without nitrite accumulation; however, the molecular pathway underlying this aerobic nitrate reduction remains unclear. In this study, we constructed a complete genome map of P. furukawaii ZS1 and performed a comparative genomic analysis with a reference strain. The results showed that P. furukawaii ZS1 genome was 6 026 050 bp in size and contained 5427 predicted protein-coding sequences. The genome contained all the necessary genes for the dissimilatory nitrate reduction to ammonia pathway but lacked those for the assimilatory nitrate reduction pathway; additionally, genes that convert ammonia to organic nitrogen were also identified. The presence of putative genes associated with the nitrogen and oxidative phosphorylation pathways implied that ZS1 can perform respiration and nitrate reduction simultaneously under aerobic conditions, so that nitrite is rapidly consumed for detoxication by denitrification. The aim of this study is to indicate the great potential of strain ZS1 for future full-scale applications in aquaculture. This work provided insights at the molecular level on the nitrogen metabolic pathways in Pseudomonas species. The understanding of nitrogen metabolic pathways also provides significant molecular information for further Pseudomonas species modification and development.

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来源期刊
Genome
Genome 生物-生物工程与应用微生物
CiteScore
5.30
自引率
3.20%
发文量
42
审稿时长
6-12 weeks
期刊介绍: Genome is a monthly journal, established in 1959, that publishes original research articles, reviews, mini-reviews, current opinions, and commentaries. Areas of interest include general genetics and genomics, cytogenetics, molecular and evolutionary genetics, developmental genetics, population genetics, phylogenomics, molecular identification, as well as emerging areas such as ecological, comparative, and functional genomics.
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