小鼠、大鼠和豚鼠在血浆、尿液和组织中牛磺酸、甜菜碱、肌氨酸和其他具有渗透活性的氨基酸水平有显著差异。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Lenka Tomasova, Klaudia Maksymiuk, Dawid Chabowski, Emilia Samborowska, Marcin Ufnal
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引用次数: 0

摘要

背景:渗透液是一种天然存在的化合物,可以保护高渗透压组织(如肾髓质)中的细胞免受渗透应激。一些氨基酸,包括牛磺酸、甜菜碱、甘氨酸、丙氨酸和肌氨酸,被认为是渗透剂。本研究旨在确定作为生物医学研究中人类疾病模型的实验动物体液和组织中这些氨基酸的水平。方法:采用液相色谱联用质谱法定量测定成年、雄性小鼠、大鼠和豚鼠血浆、尿液和组织中的牛磺酸、甘氨酸、甜菜碱、丙氨酸、β -丙氨酸和肌氨酸。结果:在分析的物种中,牛磺酸被发现在所有化合物中具有最高的组织浓度,其中心脏含量最高。豚鼠肾髓质中甜菜碱含量高于肾皮质(p < 0.01),而大鼠和小鼠肾皮质和肾髓质中甜菜碱含量无显著差异。豚鼠尿液中肌氨酸水平低于大鼠(p < 0.001),血浆中肌氨酸水平低于大鼠(p < 0.05;> 0.05),心脏(p < 0.05;< 0.05),肺(p < 0.01;< 0.01),肝脏(p < 0.001;< 0.05),肾脏(p < 0.01;< 0.01)的大鼠肌氨酸浓度显著高于小鼠和豚鼠。结论:在被调查的物种中,牛磺酸、甜菜碱和其他氨基酸的浓度存在显著差异。在为临床前研究选择动物模型时,认识到这些差异是很重要的,在为介入研究选择氨基酸剂量时,考虑到氨基酸浓度的变化也是很重要的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mice, Rats and Guinea Pigs Exhibit Significant Variations in the Plasma, Urine and Tissue Levels of Taurine, Betaine, Sarcosine and Other Osmolyte-Active Amino Acids.

Background: Osmolytes are naturally occurring compounds that protect cells from osmotic stress in high-osmolarity tissues, such as the kidney medulla. Some amino acids, including taurine, betaine, glycine, alanine, and sarcosine, are known to act as osmolytes. This study aimed to establish the levels of these amino acids in body fluids and tissues of laboratory animals used as models for human diseases in biomedical research.

Methods: Liquid chromatography coupled with mass spectrometry was used to quantify taurine, glycine, betaine, alanine, beta-alanine, and sarcosine in plasma, urine, and tissues of adult, male mice, rats and guinea pigs.

Results: Among the species analyzed, taurine was found to have the highest tissue concentrations across all compounds, with the heart containing the greatest amount. In guinea pigs, betaine levels were higher in the renal medulla than in the renal cortex (p < 0.01), while in rats and mice, there were no significant differences in betaine levels between the kidney cortex and medulla. The urine of guinea pigs had lower levels of sarcosine compared to rats (p < 0.001), while the plasma (p < 0.05; > 0.05), heart (p < 0.05; < 0.05), lungs (p < 0.01; < 0.01), liver (p < 0.001; < 0.05), and kidneys (p < 0.01; < 0.01) of rats exhibited notably higher concentrations of sarcosine compared to both mice and guinea pigs, respectively.

Conclusions: There are pronounced differences in the concentrations of taurine, betaine, and other amino acids across the investigated species. It is important to acknowledge these differences when selecting animal models for preclinical studies and to account for variations in amino acid concentrations when selecting amino acids doses for interventional studies.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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