葡萄籽原花青素提取物诱导类风湿关节炎成纤维细胞样滑膜细胞凋亡和自噬细胞死亡。

IF 1.1 4区 医学 Q4 Medicine
Ye-Rin Heo, Chang-Nam Son, Won-Ki Baek, Sang-Hyon Kim
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引用次数: 0

摘要

目的:在本研究中,我们旨在评估葡萄籽原花青素提取物(GSPE)与类风湿关节炎-成纤维细胞样滑膜细胞(RA-FLSs)的关系,并探讨GSPE是否诱导RA-FLSs细胞死亡。材料和方法:从类风湿性关节炎滑膜组织中分离FLSs。用血细胞计和流式细胞术分析细胞活力和细胞周期分期。用z-VAD-fmk进行Western blotting分析Caspase 3和聚(adp -核糖)聚合酶(PARP)蛋白。用3-甲基腺嘌呤和氯喹进行Western blot检测自噬降解的蛋白LC3和多泛素结合蛋白p62。活性氧(ROS)也进行了评估。结果:GSPE处理RA-FLSs后,细胞活力降低,g1期和G2/M期细胞数量增加,pro-PARP和pro-caspase 3蛋白表达呈浓度依赖性降低。当细胞与泛caspase抑制剂z-VAD-fmk共处理时,这一结果被抵消。当与GSPE和自噬抑制剂3-甲基腺嘌呤和氯喹共同处理RA-FLSs时,GSPE降低了细胞活力,增加了LC3-II蛋白的表达,降低了p62蛋白的表达。GSPE处理的ra - fls中ROS水平显著低于n -乙酰半胱氨酸(一种ROS抑制剂)处理的ROS水平。结论:我们的研究结果表明,GSPE可诱导RA-FLSs细胞凋亡和自噬细胞死亡,并抑制活性氧。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Grape seed proanthocyanidin extract induces apoptotic and autophagic cell death in rheumatoid arthritis fibroblast-like synoviocytes.

Grape seed proanthocyanidin extract induces apoptotic and autophagic cell death in rheumatoid arthritis fibroblast-like synoviocytes.

Grape seed proanthocyanidin extract induces apoptotic and autophagic cell death in rheumatoid arthritis fibroblast-like synoviocytes.

Grape seed proanthocyanidin extract induces apoptotic and autophagic cell death in rheumatoid arthritis fibroblast-like synoviocytes.

Objectives: In this study, we aimed to evaluate the association between grape seed proanthocyanidin extract (GSPE) and rheumatoid arthritis-fibroblast-like synoviocytes (RA-FLSs) and to investigate whether GSPE induces cell death in RA-FLSs.

Materials and methods: The FLSs were isolated from RA synovial tissues. Cell viability and cell cycle staging were analyzed using a hemocytometer and flow cytometry. Caspase 3 and poly (ADP-ribose) polymerase (PARP) proteins were analyzed using Western blotting with z-VAD-fmk. Protein LC3 and polyubiquitin-binding protein p62 that were degraded by autophagy were evaluated using Western blotting with 3-methyladenine and chloroquine. Reactive oxygen species (ROS) were also evaluated.

Results: When RA-FLSs were treated with GSPE, cell viability decreased, the number of cells in sub-G1 and G2/M phases increased, and the expression of pro-PARP and pro-caspase 3 proteins decreased in a concentration-dependent manner. This result was offset, when the cells were co-treated with the pan-caspase inhibitor z-VAD-fmk. The reduced cell viability, increased expression of LC3-II protein, and reduced expression of p62 protein with GSPE treatment were offset, when RA-FLSs were co-treated with GSPE and autophagy inhibitors 3-methyladenine and chloroquine. The level of ROS in RA-FLSs treated with GSPE was significantly lower than treatment with N-acetyl-cysteine, a ROS inhibitor.

Conclusion: Our study results show that GSPE induces apoptotic and autophagic cell death and inhibites reactive oxygen species in RA-FLSs.

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来源期刊
Archives of rheumatology
Archives of rheumatology Medicine-Rheumatology
CiteScore
2.00
自引率
9.10%
发文量
15
期刊介绍: The Archives of Rheumatology is an official journal of the Turkish League Against Rheumatism (TLAR) and is published quarterly in March, June, September, and December. It publishes original work on all aspects of rheumatology and disorders of the musculoskeletal system. The priority of the Archives of Rheumatology is to publish high-quality original research articles, especially in inflammatory rheumatic disorders. In addition to research articles, brief reports, reviews, editorials, letters to the editor can also be published. It is an independent peer-reviewed international journal printed in English. Manuscripts are refereed by a "double-blind peer-reviewed" process for both referees and authors. Editorial Board of the Archives of Rheumatology works under the principles of The World Association of Medical Editors (WAME), the International Council of Medical Journal Editors (ICMJE), and Committee on Publication Ethics (COPE).
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