{"title":"人脐带源性间充质细胞条件培养基(hMSC-CM)对四氯化碳诱导的雄性Wistar大鼠肺纤维化的恢复作用","authors":"Maryam Khajvand-Abedini, Mahdi Bahmani, Nasrin Ziamajidi, Alireza Nourian, Parisa Habibi, Shirin Heidarisasan, Roghayeh Abbasalipourkabir","doi":"10.1155/2022/7179766","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Pulmonary toxicity induced by CCl<sub>4</sub>, a model of idiopathic pulmonary fibrosis (IPF), leads to tissue remodeling and inflammation. Human umbilical cord mesenchymal cell-conditioned medium (hMSC-CM) is a potent anti-inflammatory, antioxidative, and antifibrotic agent.</p><p><strong>Methods: </strong>Forty male Wistar rats were assigned to the control (C), olive oil control (C.O) (hMSC-CM), control (C.Ms), fibrosis (fb), and fibrosis with hMSC-CM (f.Ms) treatment groups. The groups C, C.O, and C.Ms received PBS (200 <i>µ</i>l), olive oil (1 ml/kg), and hMSC-CM (100 <i>μ</i>g protein/kg), respectively. The fibrosis group was administered with only CCl4 (1 ml/kg). The last group, f.Ms was treated with CCl<sub>4</sub> (1 ml/kg) and 100 <i>μ</i>g protein/kg IV hMSC-CM. While the treatment with olive oil and CCl<sub>4</sub> was performed for 2 days/week from the first week for 12 weeks, the treatment with PBS and hMSC-CM was carried out 2 days/week from week 4<sup>th</sup> to week 12<sup>th</sup>. The effect of the UC-MSC culture medium treatment on the lung was evaluated by assessing lysyl oxidase (LOX), tumor necrosis factor-alpha (TNF-<i>α</i>), and transforming growth factor-<i>β</i>1 (TGF-<i>β</i>1) genes, and proteins expression by real-time RCR and western blotting, respectively.</p><p><strong>Results: </strong>Lysyl oxidase (LOX), tumor necrosis factor-alpha (TNF-<i>α</i>), transforming growth factor-b1 (TGF-<i>β</i>1), malondialdehyde (MDA), and oxidative stress levels were markedly higher in the fibrosis group than in the control groups (<i>p</i> ≤ 0.001). Additionally, glutathione (GSH) in the fibrosis group was markedly lower than those in the control groups (<i>p</i> ≤ 0.001). Fibrosis in the UC-MSC treatment group had milder histopathological injuries than in the fibrosis group.</p><p><strong>Conclusion: </strong>hMSC-MSC as a strong anti-inflammatory, antioxidative, and antifibrotic decreases the level of oxidative stress, proinflammatory cytokines, and MDA causing a restoring effect against CCl<sub>4</sub>-induced pulmonary fibrosis.</p>","PeriodicalId":14004,"journal":{"name":"International Journal of Inflammation","volume":"2022 ","pages":"7179766"},"PeriodicalIF":2.6000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800074/pdf/","citationCount":"0","resultStr":"{\"title\":\"The Restoring Effect of Human Umbilical Cord-Derived Mesenchymal Cell-Conditioned Medium (hMSC-CM) against Carbon Tetrachloride-Induced Pulmonary Fibrosis in Male Wistar Rats.\",\"authors\":\"Maryam Khajvand-Abedini, Mahdi Bahmani, Nasrin Ziamajidi, Alireza Nourian, Parisa Habibi, Shirin Heidarisasan, Roghayeh Abbasalipourkabir\",\"doi\":\"10.1155/2022/7179766\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Pulmonary toxicity induced by CCl<sub>4</sub>, a model of idiopathic pulmonary fibrosis (IPF), leads to tissue remodeling and inflammation. Human umbilical cord mesenchymal cell-conditioned medium (hMSC-CM) is a potent anti-inflammatory, antioxidative, and antifibrotic agent.</p><p><strong>Methods: </strong>Forty male Wistar rats were assigned to the control (C), olive oil control (C.O) (hMSC-CM), control (C.Ms), fibrosis (fb), and fibrosis with hMSC-CM (f.Ms) treatment groups. The groups C, C.O, and C.Ms received PBS (200 <i>µ</i>l), olive oil (1 ml/kg), and hMSC-CM (100 <i>μ</i>g protein/kg), respectively. The fibrosis group was administered with only CCl4 (1 ml/kg). The last group, f.Ms was treated with CCl<sub>4</sub> (1 ml/kg) and 100 <i>μ</i>g protein/kg IV hMSC-CM. While the treatment with olive oil and CCl<sub>4</sub> was performed for 2 days/week from the first week for 12 weeks, the treatment with PBS and hMSC-CM was carried out 2 days/week from week 4<sup>th</sup> to week 12<sup>th</sup>. The effect of the UC-MSC culture medium treatment on the lung was evaluated by assessing lysyl oxidase (LOX), tumor necrosis factor-alpha (TNF-<i>α</i>), and transforming growth factor-<i>β</i>1 (TGF-<i>β</i>1) genes, and proteins expression by real-time RCR and western blotting, respectively.</p><p><strong>Results: </strong>Lysyl oxidase (LOX), tumor necrosis factor-alpha (TNF-<i>α</i>), transforming growth factor-b1 (TGF-<i>β</i>1), malondialdehyde (MDA), and oxidative stress levels were markedly higher in the fibrosis group than in the control groups (<i>p</i> ≤ 0.001). Additionally, glutathione (GSH) in the fibrosis group was markedly lower than those in the control groups (<i>p</i> ≤ 0.001). Fibrosis in the UC-MSC treatment group had milder histopathological injuries than in the fibrosis group.</p><p><strong>Conclusion: </strong>hMSC-MSC as a strong anti-inflammatory, antioxidative, and antifibrotic decreases the level of oxidative stress, proinflammatory cytokines, and MDA causing a restoring effect against CCl<sub>4</sub>-induced pulmonary fibrosis.</p>\",\"PeriodicalId\":14004,\"journal\":{\"name\":\"International Journal of Inflammation\",\"volume\":\"2022 \",\"pages\":\"7179766\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800074/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Inflammation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/2022/7179766\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Inflammation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2022/7179766","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
The Restoring Effect of Human Umbilical Cord-Derived Mesenchymal Cell-Conditioned Medium (hMSC-CM) against Carbon Tetrachloride-Induced Pulmonary Fibrosis in Male Wistar Rats.
Objective: Pulmonary toxicity induced by CCl4, a model of idiopathic pulmonary fibrosis (IPF), leads to tissue remodeling and inflammation. Human umbilical cord mesenchymal cell-conditioned medium (hMSC-CM) is a potent anti-inflammatory, antioxidative, and antifibrotic agent.
Methods: Forty male Wistar rats were assigned to the control (C), olive oil control (C.O) (hMSC-CM), control (C.Ms), fibrosis (fb), and fibrosis with hMSC-CM (f.Ms) treatment groups. The groups C, C.O, and C.Ms received PBS (200 µl), olive oil (1 ml/kg), and hMSC-CM (100 μg protein/kg), respectively. The fibrosis group was administered with only CCl4 (1 ml/kg). The last group, f.Ms was treated with CCl4 (1 ml/kg) and 100 μg protein/kg IV hMSC-CM. While the treatment with olive oil and CCl4 was performed for 2 days/week from the first week for 12 weeks, the treatment with PBS and hMSC-CM was carried out 2 days/week from week 4th to week 12th. The effect of the UC-MSC culture medium treatment on the lung was evaluated by assessing lysyl oxidase (LOX), tumor necrosis factor-alpha (TNF-α), and transforming growth factor-β1 (TGF-β1) genes, and proteins expression by real-time RCR and western blotting, respectively.
Results: Lysyl oxidase (LOX), tumor necrosis factor-alpha (TNF-α), transforming growth factor-b1 (TGF-β1), malondialdehyde (MDA), and oxidative stress levels were markedly higher in the fibrosis group than in the control groups (p ≤ 0.001). Additionally, glutathione (GSH) in the fibrosis group was markedly lower than those in the control groups (p ≤ 0.001). Fibrosis in the UC-MSC treatment group had milder histopathological injuries than in the fibrosis group.
Conclusion: hMSC-MSC as a strong anti-inflammatory, antioxidative, and antifibrotic decreases the level of oxidative stress, proinflammatory cytokines, and MDA causing a restoring effect against CCl4-induced pulmonary fibrosis.
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