转基因小鼠半月板组织清除后血管分布的三维成像和定量分析。

IF 1.7 4区 生物学 Q4 CELL BIOLOGY
Huaixuan Sheng, Mingru Huang, Huizhu Li, Luyi Sun, Sijia Feng, Xiner Du, Yicong Wang, Xiaoyu Tong, Yi Feng, Jun Chen, Yunxia Li
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引用次数: 0

摘要

目的:半月板的血供决定其恢复,为制定治疗计划提供参考。本研究旨在应用组织清除和三维成像技术来探索小鼠半月板中血管的定量分布。材料与方法:本实验采用荧光血管内皮细胞清除转基因小鼠双侧膝关节组织。使用光片显微镜获取图像,并使用3D成像分析半月板中的血管内皮细胞。采用定量方法进一步分析小鼠半月板中血管的分布。结果:传统的半月板三等宽划分为:外侧三分之一为红-红区(RR),内侧三分之一是白-白区(WW),过渡区为红-白区。该划分揭示了RW和WW之间的显著信号差异(P结论:在组织清除和3D成像下对小鼠半月板的血液供应的分析反映了定量的血管分布,这将有助于未来对人类半月板的评估,并为临床医生提供更多的解剖学参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Three-Dimensional Imaging and Quantitative Analysis of Blood Vessel Distribution in The Meniscus of Transgenic Mouse after Tissue Clearing.

Three-Dimensional Imaging and Quantitative Analysis of Blood Vessel Distribution in The Meniscus of Transgenic Mouse after Tissue Clearing.

Three-Dimensional Imaging and Quantitative Analysis of Blood Vessel Distribution in The Meniscus of Transgenic Mouse after Tissue Clearing.

Three-Dimensional Imaging and Quantitative Analysis of Blood Vessel Distribution in The Meniscus of Transgenic Mouse after Tissue Clearing.

Objective: Blood supply to the meniscus determines its recovery and is a reference for treatment planning. This study aimed to apply tissue clearing and three-dimensional (3D) imaging in exploring the quantitative distribution of blood vessels in the mouse meniscus.

Materials and methods: In this experimental study, tissue clearing was performed to treat the bilateral knee joints of transgenic mice with fluorescent vascular endothelial cells. Images were acquired using a light sheet microscope and the vascular endothelial cells in the meniscus was analysed using 3D imaging. Quantitative methods were employed to further analyse the blood vessel distribution in the mouse meniscus.

Results: The traditional three-equal-width division of the meniscus is as follows: the outer one-third is the red-red zone (RR), the inner one-third is the white-white zone (WW), and the transition area is the red-white zone (RW). The division revealed significant signal differences between the RW and WW (P<0.05) zones, but no significant differences between the RR and RW zones, which indicated that the division might not accurately reflect the blood supply of the meniscus. According to the modified division (4:2:1) in which significant differences were ensured between the adjacent zones, we observed that the width ratio of each zone was 38 ± 1% (RR), 24 ± 1% (RW), and 38 ± 2% (WW). Furthermore, the blood supply to each region was verified. The anterior region had the most abundant blood supply. The fluorescence count in the anterior region was significantly higher than in the central and posterior regions (P<0.05). The blood supply of the medial meniscus was superior to the lateral meniscus (P<0.05).

Conclusion: Analysis of the blood supply to the mouse meniscus under tissue clearing and 3D imaging reflect quantitative blood vessel distribution, which would facilitate future evaluations of the human meniscus and provide more anatomical references for clinicians.

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来源期刊
Cell Journal
Cell Journal CELL BIOLOGY-
CiteScore
3.40
自引率
5.00%
发文量
0
审稿时长
12 months
期刊介绍: The “Cell Journal (Yakhteh)“, formerly published as “Yakhteh Medical Journal”, is a quarterly English publication of Royan Institute. This journal focuses on topics relevant to cellular and molecular scientific areas, besides other related fields. The Cell J has been certified by Ministry of Culture and Islamic Guidance in 1999 and was accredited as a scientific and research journal by HBI (Health and Biomedical Information) Journal Accreditation Commission in 2000 which is an open access journal.
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