以减毒鼠伤寒沙门菌为载体口服SARS-CoV-2 DNA疫苗的研究

Pub Date : 2022-01-01 DOI:10.3103/S0891416822030107
Dan Zhu, Mengyue Mengyue, Aaodeng Qimuge, Bilige Bilige, Tegexi Baiyin, Temuqile Temuqile, Shana Chen, Siqin Borjigen, Huricha Baigude, Dezhi Yang
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引用次数: 4

摘要

2019年新型冠状病毒病(COVID-19)是一种已被确定为严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)的疾病,但SARS-CoV-2的预防性治疗仍在研究中。真核表达质粒有效地传递到免疫系统的诱导细胞是产生有效DNA疫苗的基本要求。在这里,我们已经探索了使用鼠伤寒沙门氏菌作为载体,以口头传递表达质粒。采用一步基因失活法构建弱毒沙门氏菌phoP,通过电穿孔将编码SARS-CoV-2刺突蛋白的质粒转化为沙门氏菌phoP。采用Western blot实验检测SARS-CoV-2在293T细胞上的表达。用携带真核表达质粒的沙门氏菌口服免疫Wistar大鼠,每周1次,连续3周。采用ELISA法检测大鼠血清中SARS-CoV-2特异性IgG的含量。pSARS-CoV-2可在293T细胞上成功表达,所有免疫动物均对SARS-CoV-2刺突蛋白产生免疫,表明携带刺突基因的沙门氏菌疫苗可在大鼠中引发SARS-CoV-2特异性继发性免疫反应。口服使用减毒鼠伤寒沙门氏菌的SARS-CoV-2 DNA疫苗可能有助于开发针对SARS-CoV-2感染的保护性疫苗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Oral Delivery of SARS-CoV-2 DNA Vaccines Using Attenuated <i>Salmonella typhimurium</i> as a Carrier in Rat.

Oral Delivery of SARS-CoV-2 DNA Vaccines Using Attenuated <i>Salmonella typhimurium</i> as a Carrier in Rat.

Oral Delivery of SARS-CoV-2 DNA Vaccines Using Attenuated <i>Salmonella typhimurium</i> as a Carrier in Rat.

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Oral Delivery of SARS-CoV-2 DNA Vaccines Using Attenuated Salmonella typhimurium as a Carrier in Rat.

The 2019 novel coronavirus disease (COVID-19) is the disease that has been identified as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), but the prophylactic treatment of SARS-CoV-2 is still under investigation. The effective delivery of eukaryotic expression plasmids to the immune system's inductive cells constitutes an essential requirement for generating effective DNA vaccines. Here, we have explored the use of Salmonella typhimurium as vehicles to deliver expression plasmids orally. The attenuated Salmonella phoP was constructed by the one-step gene inactivation method, and plasmid-encoded the spike protein of SARS-CoV-2 was transform into the Salmonella phoP by electroporation. Western blot experiment was used for the detection of SARS-CoV-2 expression on 293T cells. Wistar rats were immunized orally with Salmonella that carried a eukaryotic expression plasmid once a week for three consecutive weeks. The ELISA was performed to measure the SARS-CoV-2 specific IgG at rat's serum samples. pSARS-CoV-2 can be successfully expression on 293T cells, and all immunized animals generated immunity against the SARS-CoV-2 spike protein, indicating that a Salmonella-based vaccine carrying the Spike gene can elicit SARS-CoV-2-specific secondary immune responses in rats. Oral delivery of SARS-CoV-2 DNA vaccines using attenuated Salmonella typhimurium may help develop a protective vaccine against SARS-CoV-2 infection.

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