从双重蛋白质组学的角度来看,感染沙门氏菌的液泡到细胞质的转变。

IF 6.7 1区 医学 Q1 Immunology and Microbiology
Ursula Fels, Patrick Willems, Margaux De Meyer, Kris Gevaert, Petra Van Damme
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引用次数: 0

摘要

通过对肠沙门氏菌血清型鼠伤寒沙门氏菌(S. Typhimurium)与其上皮宿主(这里,S. Typhimurium感染人类HeLa细胞)接触的双重蛋白质组学分析,获得了宿主-病原体在感染时间过程中相互作用的详细和整体蛋白质组学观点。基于数据独立获取(DIA)的蛋白质组学被发现优于数据依赖获取(DDA)工作流程,特别是在识别感染进展过程中下调的细菌蛋白质组反应时,通过允许在感染早期细菌感染负荷较低时定量低丰度的细菌蛋白质。鼠伤寒沙门氏菌在上皮细胞中的侵袭和复制特异性蛋白质组学特征揭示了相互依赖的宿主/病原体特异性反应,除了指向假定的新型感染标记和信号反应,包括与沙门氏菌修饰膜相关的受调节宿主蛋白。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Shift in vacuolar to cytosolic regime of infecting Salmonella from a dual proteome perspective.

Shift in vacuolar to cytosolic regime of infecting Salmonella from a dual proteome perspective.

Shift in vacuolar to cytosolic regime of infecting Salmonella from a dual proteome perspective.

Shift in vacuolar to cytosolic regime of infecting Salmonella from a dual proteome perspective.

By applying dual proteome profiling to Salmonella enterica serovar Typhimurium (S. Typhimurium) encounters with its epithelial host (here, S. Typhimurium infected human HeLa cells), a detailed interdependent and holistic proteomic perspective on host-pathogen interactions over the time course of infection was obtained. Data-independent acquisition (DIA)-based proteomics was found to outperform data-dependent acquisition (DDA) workflows, especially in identifying the downregulated bacterial proteome response during infection progression by permitting quantification of low abundant bacterial proteins at early times of infection when bacterial infection load is low. S. Typhimurium invasion and replication specific proteomic signatures in epithelial cells revealed interdependent host/pathogen specific responses besides pointing to putative novel infection markers and signalling responses, including regulated host proteins associated with Salmonella-modified membranes.

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来源期刊
PLoS Pathogens
PLoS Pathogens 生物-病毒学
CiteScore
11.40
自引率
3.00%
发文量
598
审稿时长
2 months
期刊介绍: Bacteria, fungi, parasites, prions and viruses cause a plethora of diseases that have important medical, agricultural, and economic consequences. Moreover, the study of microbes continues to provide novel insights into such fundamental processes as the molecular basis of cellular and organismal function.
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