Dan Zhao, Jinli Xiao, Lijuan Qiang, Xingwang Deng, Jingjing An, Qing Zhang, Fang Zhao, Jiaxiang Ma, Chao Fang, Guangyu Guan, Yinsheng Wu, Yan Xie
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引用次数: 2
摘要
目的:研究核桃软膏(WO)的有效成分及其修复创面的作用机制。方法:采用气相色谱-质谱联用技术对其成分进行检测。采用体外Alamar Blue (AB)试剂检测亚油酸(LA)的作用。采用Image J软件、组织学和免疫组织化学分析证实LA对猪皮肤模型的愈合作用。实验结束后，动物注射戊巴比妥钠安乐死。结果:wo24 %的LA能促进角质形成细胞和成纤维细胞的增殖，分别比对照组高50.09%和15.07% (p < 0.05)。在第3周和第4周，LA组的愈合率(96.02%±2%，98.58%±0.78%)高于生理盐水组(82.11%±3.37%，88.72%±1.73%)(p < 0.05)。与对照组相比，LA组的表皮厚度增加了0.16±0.04 mm, P63和CK10蛋白的表达增强(p < 0.05)。结论:LA是WO的主要成分，可通过刺激细胞增殖和分化促进全层烧伤创面的形成。
Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid.
Purpose: To investigate the active ingredients of walnut ointment (WO) and its mechanism in repairing wounds.
Methods: The ingredients of WO were detected by gas chromatography-mass spectrometry. The effect of linoleic acid (LA) was tested by in vitro Alamar Blue (AB) reagent. Image J software, histological and immunohistochemical analysis were used to confirm the healing effect of LA in the porcine skin model. The animals were euthanized after the experiment by injection of pentobarbital sodium.
Results: LA, 24% in WO, promotes keratinocytes and fibroblasts proliferation, which were 50.09% and 15.07% respectively higher than control (p < 0.05). The healing rate of the LA group (96.02% ± 2%, 98.58% ± 0.78%) was higher than the saline group (82.11% ± 3.37%, 88.72% ± 1.73%) at week 3 and week 4 (p < 0.05). The epidermal thickness of the LA was 0.16 ± 0.04 mm greater and the expression of the P63 and CK10 proteins was stronger in the LA group than the control (p < 0.05).
Conclusions: LA, which is the main components in WO can promote full-thickness burning wounds (FBWs) by stimulating cell proliferation and differentiation.
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