多功能纳米酶增强铜配位聚合物纳米颗粒用于耐药性细菌消除和糖尿病伤口愈合。

IF 11.3 1区 医学 Q1 Medicine
Jiahui Zhao, Tengfei Xu, Jichao Sun, Haitao Yuan, Mengyun Hou, Zhijie Li, Jigang Wang, Zhen Liang
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引用次数: 0

摘要

背景:慢性伤口中的耐药性细菌感染是一个持续存在的问题,因为它们对抗生素具有耐药性,并且由于活性氧(ROS)的产生会导致过度炎症。一个有效的解决方案不仅可以对抗细菌感染,还可以清除ROS来缓解伤口部位的炎症。具有抗氧化特性的支架因其清除ROS的能力而具有吸引力,并且在开发用于伤口愈合的抗氧化酶模拟纳米材料方面存在医学需求。方法:本研究采用自组装方法制备了铜配位聚合物纳米粒子。此外,ε-聚赖氨酸(EPL)是一种抗菌和阳离子聚合物,通过简单的一锅自组装过程整合到Cu-CPNs结构中,而不牺牲Cu-CPNs的谷胱甘肽过氧化物酶(GPx)和超氧化物歧化酶(SOD)模拟活性。结果:所制备的Cu-CPNs在模拟谷胱甘肽过氧化物酶和超氧化物歧化酶的活性方面表现出优异的抗氧化性能,并使其能够有效清除伤口部位产生的有害ROS。体外实验表明Cu-CPNs@EPL复合物具有优异的抗氧化性能和抗菌效果。细菌代谢分析表明,该复合物主要影响细胞膜的完整性和核酸合成,从而导致细菌死亡。结论:Cu-CPNs@EPL该复合物具有令人印象深刻的抗氧化性能和抗菌作用,是治疗慢性伤口耐药细菌感染的一种很有前途的解决方案。该复合物能够中和多种ROS并减少ROS诱导的炎症,有助于缓解伤口部位的炎症。ROS清除和抑菌功能的示意图Cu-CPNs@EPL纳米酶治疗MRSA感染的伤口。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Multifunctional nanozyme-reinforced copper-coordination polymer nanoparticles for drug-resistance bacteria extinction and diabetic wound healing.

Multifunctional nanozyme-reinforced copper-coordination polymer nanoparticles for drug-resistance bacteria extinction and diabetic wound healing.

Multifunctional nanozyme-reinforced copper-coordination polymer nanoparticles for drug-resistance bacteria extinction and diabetic wound healing.

Multifunctional nanozyme-reinforced copper-coordination polymer nanoparticles for drug-resistance bacteria extinction and diabetic wound healing.

Background: Drug-resistant bacterial infections in chronic wounds are a persistent issue, as they are resistant to antibiotics and can cause excessive inflammation due to generation of reactive oxygen species (ROS). An effective solution would be to not only combat bacterial infections but also scavenge ROS to relieve inflammation at the wound site. Scaffolds with antioxidant properties are attractive for their ability to scavenge ROS, and there is medical demand in developing antioxidant enzyme-mimicking nanomaterials for wound healing.

Methods: In this study, we fabricated copper-coordination polymer nanoparticles (Cu-CPNs) through a self-assembly process. Furthermore, ε-polylysine (EPL), an antibacterial and cationic polymer, was integrated into the Cu-CPNs structure through a simple one-pot self-assembly process without sacrificing the glutathione peroxidase (GPx) and superoxide dismutase (SOD)-mimicking activity of Cu-CPNs.

Results: The resulting Cu-CPNs exhibit excellent antioxidant propertiesin mimicking the activity of glutathione peroxidase and superoxide dismutase and allowing them to effectively scavenge harmful ROS produced in wound sites. The in vitro experiments showed that the resulting Cu-CPNs@EPL complex have superior antioxidant properties and antibacterial effects. Bacterial metabolic analysis revealed that the complex mainly affects the cell membrane integrity and nucleic acid synthesis that leads to bacterial death.

Conclusions: The Cu-CPNs@EPL complex has impressive antioxidant properties and antibacterial effects, making it a promising solution for treating drug-resistant bacterial infections in chronic wounds. The complex's ability to neutralize multiple ROS and reduce ROS-induced inflammation can help relieve inflammation at the wound site. Schematic illustration of the ROS scavenging and bacteriostatic function induced by Cu-CPNs@EPL nanozyme in the treatment of MRSA-infected wounds.

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来源期刊
Biomaterials Research
Biomaterials Research Medicine-Medicine (miscellaneous)
CiteScore
10.20
自引率
3.50%
发文量
63
审稿时长
30 days
期刊介绍: Biomaterials Research, the official journal of the Korean Society for Biomaterials, is an open-access interdisciplinary publication that focuses on all aspects of biomaterials research. The journal covers a wide range of topics including novel biomaterials, advanced techniques for biomaterial synthesis and fabrication, and their application in biomedical fields. Specific areas of interest include functional biomaterials, drug and gene delivery systems, tissue engineering, nanomedicine, nano/micro-biotechnology, bio-imaging, regenerative medicine, medical devices, 3D printing, and stem cell research. By exploring these research areas, Biomaterials Research aims to provide valuable insights and promote advancements in the biomaterials field.
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