María Vázquez-Reza, Antía Custodia, Iria López-Dequidt, Marta Aramburu-Núñez, Daniel Romaus-Sanjurjo, Alberto Ouro, João Botelho, Vanessa Machado, Ramón Iglesias-Rey, Juan Manuel Pías-Peleteiro, Rogelio Leira, Juan Blanco, José Castillo, Tomás Sobrino, Yago Leira
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Particularly, circulating endothelial progenitor cells (EPCs) have been considered a biomarker of altered vascular endothelial function.</p><p><strong>Objectives: </strong>The aim of this study was to investigate relationship between periodontal inflammation and increased number of circulating EPCs.</p><p><strong>Design: </strong>This is retrospective cohort study.</p><p><strong>Methods: </strong>In this study, 85 elderly patients with a previous history of hypertension were followed up to 12 months. A baseline full-mouth periodontal assessment was carried out, and the amount of periodontal tissue inflamed per subject was calculated as a proxy of periodontal inflammation [periodontal inflamed surface area (PISA)]. The number of circulating EPCs (CD34<sup>+</sup>/CD133<sup>+</sup>/KDR<sup>+</sup>) was determined by flow cytometry from peripheral blood samples collected at baseline and 12 months.</p><p><strong>Results: </strong>Mean concentrations of CD34<sup>+</sup>/CD133<sup>+</sup>/KDR<sup>+</sup> progenitor cells were higher in periodontitis patients than in those without periodontitis at baseline [55.4, 95% confidence interval (CI) = 20.8 to 90.0 <i>versus</i> 27.2, 95% CI = 13.6 to 40.8, <i>p</i> = 0.008] and 12 months (114.6, 95% CI = 53.5 to 175.7 <i>versus</i> 19.1, 95% CI = 10.8 to 27.4, <i>p</i> = 0.003). A significant increase over the follow-up was noticed in the group of subjects with periodontitis (<i>p</i> = 0.049) but not in the nonperiodontitis group (<i>p</i> = 0.819). PISA was independently associated with CD34<sup>+</sup>/CD133<sup>+</sup>/KDR<sup>+</sup> EPCs at baseline (<i>B</i> coefficient = 0.031, 95% CI = 0.005 to 0.058; <i>p</i> = 0.021). The relationship between PISA and CD34<sup>+</sup>/CD133<sup>+</sup>/KDR<sup>+</sup> EPCs at 12 months was confounded by increased baseline body mass index (<i>B</i> coefficient = 0.064, 95% CI = -0.005 to 0.132; <i>p</i> = 0.066).</p><p><strong>Conclusion: </strong>Periodontal inflammation is associated with high number of CD34<sup>+</sup>/CD133<sup>+</sup>/KDR<sup>+</sup> EPCs, thus supporting a potential link between periodontitis and endothelial dysfunction.</p>","PeriodicalId":22960,"journal":{"name":"Therapeutic Advances in Chronic Disease","volume":"14 ","pages":"20406223231178276"},"PeriodicalIF":3.3000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/46/49/10.1177_20406223231178276.PMC10285583.pdf","citationCount":"0","resultStr":"{\"title\":\"Periodontal inflammation is associated with increased circulating levels of endothelial progenitor cells: a retrospective cohort study in a high vascular risk population.\",\"authors\":\"María Vázquez-Reza, Antía Custodia, Iria López-Dequidt, Marta Aramburu-Núñez, Daniel Romaus-Sanjurjo, Alberto Ouro, João Botelho, Vanessa Machado, Ramón Iglesias-Rey, Juan Manuel Pías-Peleteiro, Rogelio Leira, Juan Blanco, José Castillo, Tomás Sobrino, Yago Leira\",\"doi\":\"10.1177/20406223231178276\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>One of the main biological mechanisms behind the link between periodontitis and atherosclerotic vascular diseases is vascular endothelial dysfunction. 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The number of circulating EPCs (CD34<sup>+</sup>/CD133<sup>+</sup>/KDR<sup>+</sup>) was determined by flow cytometry from peripheral blood samples collected at baseline and 12 months.</p><p><strong>Results: </strong>Mean concentrations of CD34<sup>+</sup>/CD133<sup>+</sup>/KDR<sup>+</sup> progenitor cells were higher in periodontitis patients than in those without periodontitis at baseline [55.4, 95% confidence interval (CI) = 20.8 to 90.0 <i>versus</i> 27.2, 95% CI = 13.6 to 40.8, <i>p</i> = 0.008] and 12 months (114.6, 95% CI = 53.5 to 175.7 <i>versus</i> 19.1, 95% CI = 10.8 to 27.4, <i>p</i> = 0.003). A significant increase over the follow-up was noticed in the group of subjects with periodontitis (<i>p</i> = 0.049) but not in the nonperiodontitis group (<i>p</i> = 0.819). PISA was independently associated with CD34<sup>+</sup>/CD133<sup>+</sup>/KDR<sup>+</sup> EPCs at baseline (<i>B</i> coefficient = 0.031, 95% CI = 0.005 to 0.058; <i>p</i> = 0.021). 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引用次数: 0
摘要
背景:牙周炎与动脉粥样硬化性血管疾病之间联系的主要生物学机制之一是血管内皮功能障碍。特别是，循环内皮祖细胞(EPCs)被认为是血管内皮功能改变的生物标志物。目的:本研究旨在探讨牙周炎症与循环内皮祖细胞数量增加之间的关系。设计:这是一项回顾性队列研究。方法:对85例有高血压病史的老年患者进行随访，随访时间为12个月。进行基线全口牙周评估，并计算每个受试者的牙周组织炎症数量作为牙周炎症的代表[牙周炎症表面积(PISA)]。循环EPCs (CD34+/CD133+/KDR+)的数量通过流式细胞术从基线和12个月时收集的外周血样本中测定。结果:在基线时，牙周炎患者的CD34+/CD133+/KDR+祖细胞的平均浓度高于无牙周炎患者[55.4,95%可信区间(CI) = 20.8 ~ 90.0 vs . 27.2, 95% CI = 13.6 ~ 40.8, p = 0.008]和12个月时(114.6,95% CI = 53.5 ~ 175.7 vs . 19.1, 95% CI = 10.8 ~ 27.4, p = 0.003)。随访发现牙周炎组明显增加(p = 0.049)，而非牙周炎组无明显增加(p = 0.819)。PISA与基线时CD34+/CD133+/KDR+ EPCs独立相关(B系数= 0.031,95% CI = 0.005 ~ 0.058;p = 0.021)。12个月时PISA与CD34+/CD133+/KDR+ EPCs之间的关系因基线体重指数增加而混淆(B系数= 0.064,95% CI = -0.005 ~ 0.132;p = 0.066)。结论:牙周炎症与大量CD34+/CD133+/KDR+ EPCs相关，因此支持牙周炎与内皮功能障碍之间的潜在联系。
Periodontal inflammation is associated with increased circulating levels of endothelial progenitor cells: a retrospective cohort study in a high vascular risk population.
Background: One of the main biological mechanisms behind the link between periodontitis and atherosclerotic vascular diseases is vascular endothelial dysfunction. Particularly, circulating endothelial progenitor cells (EPCs) have been considered a biomarker of altered vascular endothelial function.
Objectives: The aim of this study was to investigate relationship between periodontal inflammation and increased number of circulating EPCs.
Design: This is retrospective cohort study.
Methods: In this study, 85 elderly patients with a previous history of hypertension were followed up to 12 months. A baseline full-mouth periodontal assessment was carried out, and the amount of periodontal tissue inflamed per subject was calculated as a proxy of periodontal inflammation [periodontal inflamed surface area (PISA)]. The number of circulating EPCs (CD34+/CD133+/KDR+) was determined by flow cytometry from peripheral blood samples collected at baseline and 12 months.
Results: Mean concentrations of CD34+/CD133+/KDR+ progenitor cells were higher in periodontitis patients than in those without periodontitis at baseline [55.4, 95% confidence interval (CI) = 20.8 to 90.0 versus 27.2, 95% CI = 13.6 to 40.8, p = 0.008] and 12 months (114.6, 95% CI = 53.5 to 175.7 versus 19.1, 95% CI = 10.8 to 27.4, p = 0.003). A significant increase over the follow-up was noticed in the group of subjects with periodontitis (p = 0.049) but not in the nonperiodontitis group (p = 0.819). PISA was independently associated with CD34+/CD133+/KDR+ EPCs at baseline (B coefficient = 0.031, 95% CI = 0.005 to 0.058; p = 0.021). The relationship between PISA and CD34+/CD133+/KDR+ EPCs at 12 months was confounded by increased baseline body mass index (B coefficient = 0.064, 95% CI = -0.005 to 0.132; p = 0.066).
Conclusion: Periodontal inflammation is associated with high number of CD34+/CD133+/KDR+ EPCs, thus supporting a potential link between periodontitis and endothelial dysfunction.
期刊介绍：
Therapeutic Advances in Chronic Disease publishes the highest quality peer-reviewed research, reviews and scholarly comment in the drug treatment of all chronic diseases. The journal has a strong clinical and pharmacological focus and is aimed at clinicians and researchers involved in the medical treatment of chronic disease, providing a forum in print and online for publishing the highest quality articles in this area.
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