加大对大肠杆菌基本基因的研究力度。

IF 8.5 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Systems Biology Pub Date : 2023-10-12 Epub Date: 2023-09-18 DOI:10.15252/msb.202311933

Arun Kumar, Peter C Stirling

引用次数: 0

摘要

温度敏感（TS）等位基因产生可调节的热开关，以耗尽必要的细胞活性，并用于剖析基因功能。在他们最近的研究中，Link及其同事（Schramm等人，2023）使用基于CRISPR的方法，在大肠杆菌中系统地创建跨必需基因的TS等位基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Turning up the heat on essential E. coli genes.

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Turning up the heat on essential E. coli genes.

Temperature-sensitive (TS) alleles create tunable thermoswitches to deplete essential cellular activities and are used to dissect gene function. In their recent study, Link and colleagues (Schramm et al 2023) use a CRISPR-based approach to systematically create TS alleles across essential genes in E. coli.

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来源期刊

Molecular Systems Biology 生物-生化与分子生物学

CiteScore

18.50

自引率

1.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Systems biology is a field that aims to understand complex biological systems by studying their components and how they interact. It is an integrative discipline that seeks to explain the properties and behavior of these systems. Molecular Systems Biology is a scholarly journal that publishes top-notch research in the areas of systems biology, synthetic biology, and systems medicine. It is an open access journal, meaning that its content is freely available to readers, and it is peer-reviewed to ensure the quality of the published work.