纳米银修饰细胞外基质移植物的制备及肌腱重建性能。

IF 11.3 1区 医学 Q1 Medicine
Sunfang Chen, Dan Cai, Qi Dong, Gaoxiang Ma, Chennan Xu, Xiaogang Bao, Wei Yuan, Bing Wu, Bin Fang
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引用次数: 0

摘要

背景:大缺损肌腱的重建需要高机械强度的移植物,并且经常受到感染和粘连等并发症的阻碍。因此,结合机械弹性和抗菌/抗粘连活性优点的移植物受到高度追捧。方法:将没食子酸和硝酸银合成的纳米银(GA-AgNPs)附着于脱细胞的细胞外基质(脱细胞肌腱交联GA-AgNPs, DT-Ag)。我们检测了DT-Ag的组织结构、力学性能、形态、Zeta电位、细胞毒性、抗菌性能、抗氧化和抗炎性能以及治疗动物肌腱缺损的能力。结果:每50 mg DT携带约108.57±0.94 μg GA-Ag NPs,交联部分GA-Ag NPs为65.47±0.57%,DT- ag具有持久的抗菌活性。同时,GA赋予DT-Ag良好的抗氧化和抗炎活性。此外,DT-Ag促进M2巨噬细胞极化,并通过阻碍成纤维细胞粘附抑制纤维蛋白沉积。此外,DT-Ag的主要优势是其持久的抗菌活性(以大肠杆菌和金黄色葡萄球菌为模型进行测试)和防止组织粘连的能力在体内得到了证实。结论:制备的多功能肌腱移植物具有良好的亲水性、生物相容性和机械弹性,适合处理外科肌腱重建的主要并发症,具有良好的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Silver nanoparticles-decorated extracellular matrix graft: fabrication and tendon reconstruction performance.

Silver nanoparticles-decorated extracellular matrix graft: fabrication and tendon reconstruction performance.

Silver nanoparticles-decorated extracellular matrix graft: fabrication and tendon reconstruction performance.

Silver nanoparticles-decorated extracellular matrix graft: fabrication and tendon reconstruction performance.

Background: The reconstruction of tendons with large defects requires grafts with high mechanical strength and is often hindered by complications such as infection and adhesion. Hence, grafts combining the advantages of mechanical resilience and antibacterial/antiadhesion activity are highly sought after.

Methods: The silver nanoparticles (GA-Ag NPs) synthesized from gallic acid and silver nitrate were attached to a decellularized extracellular matrix (Decellularized Tendon crosslinking GA-AgNPs, DT-Ag). We examined the histological structure, mechanical property, morphology, Zeta potential, cytotoxicity, antibacterial properties, antioxidant and anti-inflammatory properties, and ability of the DT-Ag to treat tendon defects in animals.

Results: Approximately 108.57 ± 0.94 μg GA-Ag NPs loaded per 50 mg DT, the cross-linked part of GA-Ag NPs was 65.47 ± 0.57%, which provided DT-Ag with long-lasting antibacterial activity. Meanwhile, GA endowed DT-Ag with good antioxidant and anti-inflammatory activities. Additionally, The DT-Ag facilitated M2 macrophage polarization, and suppressed fibrin deposition by hindering fibroblast adhesion. Mormore, the main advantages of DT-Ag, namely its long-lasting antibacterial activity (tested using Escherichia coli and Staphylococcus aureus as models) and the ability to prevent tissue adhesion were confirmed in vivo.

Conclusion: The fabricated multifunctional tendon graft was highly hydrophilic, biocompatible, and mechanically resilient, and concluded to be well suited for dealing with the main complications of surgical tendon reconstruction and has bright application prospects.

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来源期刊
Biomaterials Research
Biomaterials Research Medicine-Medicine (miscellaneous)
CiteScore
10.20
自引率
3.50%
发文量
63
审稿时长
30 days
期刊介绍: Biomaterials Research, the official journal of the Korean Society for Biomaterials, is an open-access interdisciplinary publication that focuses on all aspects of biomaterials research. The journal covers a wide range of topics including novel biomaterials, advanced techniques for biomaterial synthesis and fabrication, and their application in biomedical fields. Specific areas of interest include functional biomaterials, drug and gene delivery systems, tissue engineering, nanomedicine, nano/micro-biotechnology, bio-imaging, regenerative medicine, medical devices, 3D printing, and stem cell research. By exploring these research areas, Biomaterials Research aims to provide valuable insights and promote advancements in the biomaterials field.
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