花生四烯酸对体外孵化前后牛胚胎发育的影响。

IF 1.8 4区生物学 Q3 DEVELOPMENTAL BIOLOGY

Reproduction, fertility, and development Pub Date : 2023-07-01 DOI:10.1071/RD23053

L González-Brusi, A Pérez-Gómez, A C Quiroga, C Tamargo, P Ramos-Ibeas, P Bermejo-Álvarez

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引用次数: 0

摘要

背景:花生四烯酸(AA)是前列腺素的前体，在胚胎早期发育过程中可能起自分泌作用。目的:研究在孵育前后培养基中添加AA对体外培养牛胚胎发育的影响。方法:用100 μ m和333μM AA的人工输卵管液培养牛受精卵，观察AA的预孵化效果。通过在N2B27中添加5、10、20或100μM AA，培养第7天囊胚至第12天，观察AA的孵化后效果。关键结果:在333μM AA下，囊胚的预孵化发育完全消失，而在100μM AA下，囊胚率和细胞数量没有改变。在100μM AA下观察到孵化后发育受损，而在5、10和20μM AA下没有观察到存活率的影响。然而，在10和20μM AA下，第12天的胚胎大小明显减小。5-10μM AA对下胚层迁移、外胚层存活及胚盘状结构的形成均无影响。AA暴露使第12天胚胎的PTGIS、PPARG、LDHA和SCD基因下调。结论:AA对未孵化的胚胎大多无反应，而AA对孵化后早期发育有负面影响。提示:AA不能改善体外牛胚胎发育，并且在早期孵化后阶段不需要。

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Effect of arachidonic acid on pre- and post-hatching in vitro bovine embryo development.

Context: Arachidonic acid (AA) is the precursor of prostaglandins, which may play autocrine roles during early embryo development.

Aims: To test the developmental effects of addition of AA to pre- and post-hatching culture media on in vitro -produced bovine embryos.

Methods: Pre-hatching effects of AA were tested by culturing bovine zygotes in synthetic oviductal fluid (SOF) supplemented with 100 or 333μM AA. Post-hatching effects of AA were tested by culturing Day 7 blastocysts in N2B27 supplemented with 5, 10, 20 or 100μM AA up to Day 12.

Key results: Pre-hatching development to blastocyst was completely abrogated at 333μM AA, whereas blastocyst rates and cell numbers were not altered at 100μM AA. Impaired post-hatching development was observed at 100μM AA, whereas no effect on survival rates was noted at 5, 10 and 20μM AA. However, a significant reduction in Day 12 embryo size was observed at 10 and 20μM AA. Hypoblast migration, epiblast survival and formation of embryonic-disc-like structures were unaffected at 5-10μM AA. AA exposure downregulated the genes PTGIS , PPARG , LDHA and SCD in Day 12 embryos.

Conclusions: Pre-hatching embryos are mostly irresponsive to AA, whereas AA was observed to have negative effects during early post-hatching development.

Implications: AA does not improve in vitro bovine embryo development and is not required up to early post-hatching stages.

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来源期刊

Reproduction, fertility, and development 生物-动物学

CiteScore

2.10

自引率

10.50%

发文量

317

审稿时长

2 months

期刊介绍： Reproduction, Fertility and Development is an international journal for the publication of original and significant contributions on vertebrate reproductive and developmental biology. Subject areas include, but are not limited to: physiology, biochemistry, cell and molecular biology, endocrinology, genetics and epigenetics, behaviour, immunology and the development of reproductive technologies in humans, livestock and wildlife, and in pest management. Reproduction, Fertility and Development is a valuable resource for research scientists working in industry or academia on reproductive and developmental biology, clinicians and veterinarians interested in the basic science underlying their disciplines, and students. Reproduction, Fertility and Development is the official journal of the International Embryo Technology Society and the Society for Reproductive Biology. Reproduction, Fertility and Development is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.