光遗传诱导哺乳动物细胞系焦亡、坏死和凋亡的研究。

Kateryna Shkarina, Petr Broz
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引用次数: 0

摘要

受调节的细胞死亡在免疫、发育和体内平衡中起着关键作用,但也与许多病理如自身炎症、神经退行性疾病和癌症有关。然而,尽管对不同的细胞死亡方式进行了广泛的机制研究,但对不同形式的细胞死亡及其在细胞和组织水平上的后果的直接比较仍然缺乏特征。细胞死亡模式之间的机制和动力学差异,以及无法在细胞群体或组织中的单个细胞中选择性地诱导不同的细胞死亡程序,阻碍了比较研究。在这种方法中,我们提出了一种快速和特异性的光遗传激活三种主要类型的程序性细胞死亡的方案:凋亡,坏死坏死和焦亡,使用光诱导其主要效应蛋白(半胱天冬酶或激酶)的强制寡聚化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Optogenetic Induction of Pyroptosis, Necroptosis, and Apoptosis in Mammalian Cell Lines.

Optogenetic Induction of Pyroptosis, Necroptosis, and Apoptosis in Mammalian Cell Lines.

Optogenetic Induction of Pyroptosis, Necroptosis, and Apoptosis in Mammalian Cell Lines.

Optogenetic Induction of Pyroptosis, Necroptosis, and Apoptosis in Mammalian Cell Lines.

Regulated cell death plays a key role in immunity, development, and homeostasis, but is also associated with a number of pathologies such as autoinflammatory and neurodegenerative diseases and cancer. However, despite the extensive mechanistic research of different cell death modalities, the direct comparison of different forms of cell death and their consequences on the cellular and tissue level remain poorly characterized. Comparative studies are hindered by the mechanistic and kinetic differences between cell death modalities, as well as the inability to selectively induce different cell death programs in an individual cell within cell populations or tissues. In this method, we present a protocol for rapid and specific optogenetic activation of three major types of programmed cell death: apoptosis, necroptosis, and pyroptosis, using light-induced forced oligomerization of their major effector proteins (caspases or kinases).

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