附加染色在评估腹膜回归分级评分(PRGS)在胃源性腹膜转移中的作用。

IF 1.3 4区 医学 Q3 ANATOMY & MORPHOLOGY
Wiebke Solass, Giorgi Nadiradze, Marc A Reymond, Hans Bösmüller
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引用次数: 1

摘要

引言:腹膜回归分级评分(PRGS)是一种4级组织学回归分级评分,用于确定腹膜转移对化疗的反应。建议在每个腹部象限进行腹膜活检。阳性治疗反应被定义为2个治疗周期之间的平均PRGS降低或稳定。周期性酸性缎(PAS)和Ber-EP4染色对诊断PRGS1(缺乏重要肿瘤细胞)的附加值与HE染色相比尚不清楚。材料与方法:对33例癌症腹膜转移患者76次腹腔镜手术中339例活检标本进行分析。分类为PRGS1(无残留肿瘤,n=95)或不确定(n=50)的活检用PAS染色,其余不确定或PRGS1病例用BerEP4额外染色。结果:在145例活检中有28例(19%)检测到PAS染色的肿瘤细胞后,其余117例活检用Ber-EP4免疫染色。在22例活检中检测到肿瘤细胞(19%)。总的来说,额外的染色使339例活检中的50例(15%)能够检测到残留的肿瘤细胞,并改变了33例患者中的7例(21%)的治疗反应评估。结论:总之,在用PAS和/或BerEp4进行额外染色后,25%(95个中的24个)的最初无肿瘤样本(PRGS1)显示出残留的肿瘤细胞。免疫组织化学在339例活检中的22例(11.2%)提供了重要的额外信息(肿瘤细胞的存在)。进一步的染色将不清楚诊断的情况从50例减少到0例,并改变了33例患者中7例(21%)的治疗反应评估。我们建议在PRGS1或不清楚的情况下进行额外染色。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Role of Additional Staining in the Assessment of the Peritoneal Regression Grading Score (PRGS) in Peritoneal Metastasis of Gastric Origin.

Introduction: The Peritoneal Regression Grading Score (PRGS) is a 4-tied histologic regression grading score for determining the response of peritoneal metastasis to chemotherapy. Peritoneal biopsies in every abdominal quadrant are recommended. A positive therapy response is defined as a decreasing or stable mean PRGS between 2 therapy cycles. The added value of periodic acid satin (PAS) and Ber-EP4 staining over HE staining for diagnosing PRGS1 (the absence of vital tumor cells) is unclear.

Materials and methods: A total of 339 biopsies obtained during 76 laparoscopies in 33 patients with peritoneal metastasis of gastric cancer were analyzed. Biopsies classified as PRGS 1 (no residual tumor, n=95) or indefinite (n=50) were stained with PAS, and remaining indefinite or PRGS1 cases additionally stained with BerEP4.

Results: After PAS-staining tumor cells were detected in 28 out of 145 biopsies (19%), the remaining 117 biopsies were immunostained with Ber-EP4. Tumor cells were detected in 22 biopsies (19%). In total, additional staining allowed the detection of residual tumor cells in 50 out of 339 biopsies (15%) and changed the therapy response assessment in 7 out of 33 (21%) patients.

Conclusions: In summary, 25% (24 out of 95) of initially tumor-free samples (PRGS1) showed residual tumor cells after additional staining with PAS and/or BerEp4. Immunohistochemistry provided important additional information (the presence of tumor cells) in 22 of all 339 biopsies (11.2%). Further staining reduced the instances of unclear diagnosis from 50 to 0 and changed the therapy response assessment in 7 out of 33 patients (21%). We recommend additional staining in PRGS1 or unclear cases.

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来源期刊
Applied Immunohistochemistry & Molecular Morphology
Applied Immunohistochemistry & Molecular Morphology ANATOMY & MORPHOLOGY-MEDICAL LABORATORY TECHNOLOGY
CiteScore
3.20
自引率
0.00%
发文量
153
期刊介绍: ​Applied Immunohistochemistry & Molecular Morphology covers newly developed identification and detection technologies, and their applications in research and diagnosis for the applied immunohistochemist & molecular Morphologist. Official Journal of the International Society for Immunohistochemisty and Molecular Morphology​.
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