婴儿脂肪和脐带间充质干细胞的多系分化潜力。

IF 1.9 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL
Hui-Kuang Huang, Kuang-Kai Hsueh, Yu-Ting Liao, Szu-Hsien Wu, Po-Hsin Chou, Shih-Han Yeh, Jung-Pan Wang
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引用次数: 0

摘要

背景:本研究旨在比较来自切除多趾脂肪组织的婴儿脂肪源性间充质干细胞(ADSCs)和脐带源性间充质干细胞(UCSCs)在增殖和分化能力方面的生物学特性。通过测定细胞数目的倍数变化和倍增时间,分析了婴儿ADSCs和UCSCs的增殖情况。方法:通过分析衰老相关基因表达、衰老相关β-半乳糖苷酶(SA-β-gal)染色、磷酸化组蛋白变异体H2AX (γH2AX)免疫荧光染色,比较衰老和复制应激状态。采用逆转录-定量聚合酶链式反应(RT-qPCR)分析超氧化物歧化酶(sod)和多系分化相关基因的表达水平。采用组织化学染色、免疫组织化学染色和免疫荧光染色测定分化水平。结果:与UCSCs相比,婴儿ADSCs在3-5代时具有更高的增殖率和SOD1、SOD2和SOD3的表达水平。衰老相关基因(p16、p21和p53)、SA-β-gal染色和复制应激分析在婴儿ADSCs中减少。婴儿adsc分化细胞中软骨形成基因COL2、COL10、成骨基因RUNX2、ALP、脂肪形成基因LPL、肝形成基因ALB、TAT的表达水平显著高于ucsc。组织化学和免疫荧光染色证实了这些结果。婴儿adsc分化细胞中只有MMP3、DCN和COL3的表达水平低于ucsc。结论:与UCSCs相比,婴儿ADSCs具有更高的增殖率,更少的细胞衰老和复制应激,更强的抗氧化活性,更强的向软骨、成骨、脂肪和肝源谱系分化的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Multilineage differentiation potential in the infant adipose- and umbilical cord-derived mesenchymal stem cells.

Background: This study aims to compare the biological properties of infant adipose-derived mesenchymal stem cells (infant ADSCs) from excised polydactyly fat tissue and umbilical cord-derived mesenchymal stem cells (UCSCs) in terms of proliferation and differentiation capabilities. The proliferation of infant ADSCs and UCSCs was analyzed by determining the fold changes of cell numbers and doubling time periods.

Methods: The state of senescence and replicative stress was compared by analyzing the expression of age-related genes, senescence-associated β-galactosidase (SA-β-gal) staining, and phosphorylated histone variant H2AX (γH2AX) immunofluorescence staining. The expression levels of superoxide dismutase ( SODs ) and genes related to multilineage differentiation were analyzed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Differentiation levels were determined using histochemical staining, immunohistochemical staining, and immunofluorescence staining.

Results: Infant ADSCs exhibited higher proliferation rates and expression levels of SOD1 , SOD2 , and SOD3 at passages 3-5 compared with UCSCs. Senescence related genes ( p16 , p21 , and p53 ), SA-β-gal staining, and replicative stress analysis were reduced in infant ADSCs. The expression levels of chondrogenic genes ( COL2 and COL10 ), osteogenic genes ( RUNX2 and ALP ), adipogenic genes ( LPL ), and hepatogenic genes ( ALB and TAT ) in infant ADSC-differentiated cells were significantly higher than those in UCSCs. Histochemical and immunofluorescence staining confirmed these results. Only the expression levels of tenogenic genes ( MMP3 , DCN , and COL3 ) in infant ADSC-differentiated cells were lower than those in UCSCs.

Conclusion: Infant ADSCs exhibit higher proliferation rates, reduced cellular senescence and replicative stress, better antioxidative activity, and higher differentiation potential toward chondrogenic, osteogenic, adipogenic and hepatogenic lineages than UCSCs.

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来源期刊
Journal of the Chinese Medical Association
Journal of the Chinese Medical Association MEDICINE, GENERAL & INTERNAL-
CiteScore
6.20
自引率
13.30%
发文量
320
审稿时长
15.5 weeks
期刊介绍: Journal of the Chinese Medical Association, previously known as the Chinese Medical Journal (Taipei), has a long history of publishing scientific papers and has continuously made substantial contribution in the understanding and progress of a broad range of biomedical sciences. It is published monthly by Wolters Kluwer Health and indexed in Science Citation Index Expanded (SCIE), MEDLINE®, Index Medicus, EMBASE, CAB Abstracts, Sociedad Iberoamericana de Informacion Cientifica (SIIC) Data Bases, ScienceDirect, Scopus and Global Health. JCMA is the official and open access journal of the Chinese Medical Association, Taipei, Taiwan, Republic of China and is an international forum for scholarly reports in medicine, surgery, dentistry and basic research in biomedical science. As a vehicle of communication and education among physicians and scientists, the journal is open to the use of diverse methodological approaches. Reports of professional practice will need to demonstrate academic robustness and scientific rigor. Outstanding scholars are invited to give their update reviews on the perspectives of the evidence-based science in the related research field. Article types accepted include review articles, original articles, case reports, brief communications and letters to the editor
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