量化转录组多样性:综述。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Emma F Jones, Anisha Haldar, Vishal H Oza, Brittany N Lasseigne
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引用次数: 0

摘要

根据分子生物学的核心教条,基因表达异质性有助于预测和解释各种蛋白质产物、功能以及最终的表型异质性。目前用于描述基因表达谱多样性类型的术语存在重叠,忽略这些细微差别可能会错误地反映重要的生物学信息。在此,我们将转录组多样性描述为衡量以下方面异质性的一种方法:(1)一个样本中所有基因的表达,或一个群体中不同样本中单个基因的表达(基因水平多样性),或(2)给定基因的同工酶特异性表达(同工酶水平多样性)。我们首先概述了基因水平转录组多样性的调节因子和量化方法。然后,我们将讨论替代剪接在推动转录本同工酶水平多样性方面所起的作用,以及如何对其进行量化。此外,我们还概述了计算高通量测序数据的基因水平和同工酶水平多样性的计算资源。最后,我们讨论了转录组多样性的未来应用。本综述全面概述了基因表达多样性是如何产生的,以及如何通过测量基因表达多样性来更全面地了解蛋白质、细胞、组织、生物体和物种之间的异质性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quantifying transcriptome diversity: a review.

Following the central dogma of molecular biology, gene expression heterogeneity can aid in predicting and explaining the wide variety of protein products, functions and, ultimately, heterogeneity in phenotypes. There is currently overlapping terminology used to describe the types of diversity in gene expression profiles, and overlooking these nuances can misrepresent important biological information. Here, we describe transcriptome diversity as a measure of the heterogeneity in (1) the expression of all genes within a sample or a single gene across samples in a population (gene-level diversity) or (2) the isoform-specific expression of a given gene (isoform-level diversity). We first overview modulators and quantification of transcriptome diversity at the gene level. Then, we discuss the role alternative splicing plays in driving transcript isoform-level diversity and how it can be quantified. Additionally, we overview computational resources for calculating gene-level and isoform-level diversity for high-throughput sequencing data. Finally, we discuss future applications of transcriptome diversity. This review provides a comprehensive overview of how gene expression diversity arises, and how measuring it determines a more complete picture of heterogeneity across proteins, cells, tissues, organisms and species.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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