希腊HCV感染患者维生素D受体基因多态性与纤维化易感性之间的关系

IF 1.7 Q3 INFECTIOUS DISEASES
GERMS Pub Date : 2022-09-01 DOI:10.18683/germs.2022.1342
Angeliki Anna Beka, Vasileios Papadopoulos, Theodora Mylopoulou, Maria Panopoulou, Ioannis Karakasiliotis, Penelope Mavromara, Konstantinos Mimidis, Stavroula Veletza
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引用次数: 0

摘要

丙型肝炎病毒(HCV)感染是世界范围内慢性肝炎的主要原因,通常悄无声息地发展为纤维化、肝硬化和肝细胞癌(HCC)。值得注意的是,大多数感染HCV的个体在晚期才出现症状,通常伴有肝损伤,在病毒清除后无法恢复。因此,目前的抗病毒治疗不足以在不久的将来消除丙型肝炎病毒的全球负担。在过去的几年中,维生素D缺乏以及维生素D受体(VDR)基因中的某些单核苷酸多态性与肝纤维化有关。因此,本研究的目的是探讨VDR多态性ApaI (rs7975232)和TaqI (rs731236)与希腊色雷斯HCV感染患者纤维化阶段之间的可能相关性。方法:81例HCV感染患者采用瞬时弹性成像评估其纤维化分期,并采用pcr -限制性片段长度多态性(RFLP)基因分型检测VDR、ApaI和TaqI多态性。然后使用有序回归模型将VDR基因型与患者的纤维化分期进行统计学关联。结果:非肝硬化分期与TaqI TT基因型呈正相关(p=0.003),与TaqI TC基因型呈负相关(p=0.007)。在两种VDR多态性存在Hardy-Weinberg平衡和连锁不平衡的情况下,轻度纤维化分期(F0-2)与ApaI/TaqI GG/TT (p=0.002)和TG/TT (p=0.008)基因型相关,而肝硬化F4期与ApaI/TaqI TG/TC基因型相关(p=0.038)。结论:TaqI TT、ApaI/TaqI GG/TT、TG/TT和TG/TC基因型可作为HCV患者纤维化易感性的预后遗传标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Association between vitamin D receptor gene polymorphisms and fibrosis susceptibility in Greek patients with HCV infection.

Introduction: Hepatitis C virus (HCV) infection is a prime cause of chronic hepatitis worldwide, that often silently progresses to fibrosis, cirrhosis and hepatocellular carcinoma (HCC). Notably, the majority of individuals infected with HCV develop symptoms at late stages, often associated with liver damage that cannot revert after virus clearance. Thus, current antiviral therapy alone is rather insufficient to eliminate the global burden of HCV in the near future.During the past few years, vitamin D deficiency as well as certain single nucleotide polymorphisms in the vitamin D receptor (VDR) gene have been associated with liver fibrosis. Therefore, the aim of the present study was to investigate the possible correlation between VDR polymorphisms ApaI (rs7975232) and TaqI (rs731236) and the fibrosis stage of patients with HCV infection from Thrace, Greece.

Methods: Eighty-one patients with HCV infection underwent transient elastography for the assessment of their fibrosis stage, and PCR-restriction fragment length polymorphism (RFLP) genotyping for VDR ApaI and TaqI polymorphisms. VDR genotypes were then statistically associated with the patients' fibrosis stage using ordinal regression models.

Results: Non-cirrhotic stages were positively correlated with TaqI TT genotype (p=0.003) and negatively correlated with TaqI TC genotype (p=0.007). In the presence of Hardy-Weinberg equilibrium and linkage disequilibrium between the two VDR polymorphisms, mild fibrosis stages (F0-2) were correlated with ApaI/TaqI GG/TT (p=0.002) and TG/TT (p=0.008) genotypes, while cirrhotic stage F4 was associated with ApaI/TaqI TG/TC genotype (p=0.038).

Conclusions: TaqI TT and ApaI/TaqI GG/TT, TG/TT and TG/TC genotypes could be explored as prognostic genetic markers for fibrosis susceptibility in HCV patients.

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来源期刊
GERMS
GERMS INFECTIOUS DISEASES-
CiteScore
2.80
自引率
5.00%
发文量
36
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