SARS-CoV-2感染诱导内源性逆转录病毒LTR69亚家族的表观遗传变化。

IF 4.7 2区 生物学 Q1 GENETICS & HEREDITY
Ankit Arora, Jan Eric Kolberg, Smitha Srinivasachar Badarinarayan, Natalia Savytska, Daksha Munot, Martin Müller, Veronika Krchlíková, Daniel Sauter, Vikas Bansal
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引用次数: 0

摘要

越来越多的证据表明,内源性逆转录病毒(erv)在宿主对感染的反应和疾病的发展中起着重要作用。通过分析chip测序数据集,我们发现SARS-CoV-2感染诱导erv LTR69亚家族中几个位点的H3K27乙酰化。通过功能分析,我们确定了一个sars - cov -2激活的LTR69位点,称为Dup69,它具有调节活性,并对转录因子IRF3和p65/RELA有反应。LTR69_Dup69位于长链非编码RNA基因(ENSG00000289418)上游约500 bp,位于编码糖尿病相关自身抗原的PTPRN2基因内。ENSG00000289418和PTPRN2在SARS-CoV-2感染后表达显著增加。因此,我们的研究揭示了外源性病毒与内源性病毒的相互作用,并有助于理解erv在感染过程中如何调节基因表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

SARS-CoV-2 infection induces epigenetic changes in the LTR69 subfamily of endogenous retroviruses.

SARS-CoV-2 infection induces epigenetic changes in the LTR69 subfamily of endogenous retroviruses.

SARS-CoV-2 infection induces epigenetic changes in the LTR69 subfamily of endogenous retroviruses.

Accumulating evidence suggests that endogenous retroviruses (ERVs) play an important role in the host response to infection and the development of disease. By analyzing ChIP-sequencing data sets, we show that SARS-CoV-2 infection induces H3K27 acetylation of several loci within the LTR69 subfamily of ERVs. Using functional assays, we identified one SARS-CoV-2-activated LTR69 locus, termed Dup69, which exhibits regulatory activity and is responsive to the transcription factors IRF3 and p65/RELA. LTR69_Dup69 is located about 500 bp upstream of a long non-coding RNA gene (ENSG00000289418) and within the PTPRN2 gene encoding a diabetes-associated autoantigen. Both ENSG00000289418 and PTPRN2 showed a significant increase in expression upon SARS-CoV-2 infection. Thus, our study sheds light on the interplay of exogenous with endogenous viruses and helps to understand how ERVs regulate gene expression during infection.

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来源期刊
Mobile DNA
Mobile DNA GENETICS & HEREDITY-
CiteScore
8.20
自引率
6.10%
发文量
26
审稿时长
11 weeks
期刊介绍: Mobile DNA is an online, peer-reviewed, open access journal that publishes articles providing novel insights into DNA rearrangements in all organisms, ranging from transposition and other types of recombination mechanisms to patterns and processes of mobile element and host genome evolution. In addition, the journal will consider articles on the utility of mobile genetic elements in biotechnological methods and protocols.
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