W. Knapp , H. Ludwig, G. Schernthaner, R. Höfer, C. Steffen
{"title":"3 .用抗原病毒链证明百里菌素病毒","authors":"W. Knapp , H. Ludwig, G. Schernthaner, R. Höfer, C. Steffen","doi":"10.1016/S0300-872X(76)80050-9","DOIUrl":null,"url":null,"abstract":"<div><p>Previous investigations showed that quantitative immunofluorescence using antigens covalently bound to agarose particles represents a reproducible and sensitive assay for antibodies in experimental antisera. In this paper data are presented which show that also thyroglobulin antibodies in patients sera can be demonstrated using the defined antigen substrate spheres (DASS) system with thyroglobulin as antigen. Sera of 45 patients with various thyroid diseases were investigated for the presence of thyroglobulin antibodies using passive haemagglutination and the quantitative immunofluorescence technique. Comparable results were obtained with both techniques. Advantages and disadvantages of both methods are discussed.</p></div>","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"151 1","pages":"Pages 46-60"},"PeriodicalIF":0.0000,"publicationDate":"1976-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80050-9","citationCount":"0","resultStr":"{\"title\":\"Mikrofluorometrischer Nachweis von Thyreoglobulin-Antikörpern mit dem definierten Antigen-Substrat-System (DASS)1)\",\"authors\":\"W. Knapp , H. Ludwig, G. Schernthaner, R. Höfer, C. Steffen\",\"doi\":\"10.1016/S0300-872X(76)80050-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Previous investigations showed that quantitative immunofluorescence using antigens covalently bound to agarose particles represents a reproducible and sensitive assay for antibodies in experimental antisera. In this paper data are presented which show that also thyroglobulin antibodies in patients sera can be demonstrated using the defined antigen substrate spheres (DASS) system with thyroglobulin as antigen. Sera of 45 patients with various thyroid diseases were investigated for the presence of thyroglobulin antibodies using passive haemagglutination and the quantitative immunofluorescence technique. Comparable results were obtained with both techniques. Advantages and disadvantages of both methods are discussed.</p></div>\",\"PeriodicalId\":101289,\"journal\":{\"name\":\"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie\",\"volume\":\"151 1\",\"pages\":\"Pages 46-60\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1976-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80050-9\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0300872X76800509\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0300872X76800509","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Mikrofluorometrischer Nachweis von Thyreoglobulin-Antikörpern mit dem definierten Antigen-Substrat-System (DASS)1)
Previous investigations showed that quantitative immunofluorescence using antigens covalently bound to agarose particles represents a reproducible and sensitive assay for antibodies in experimental antisera. In this paper data are presented which show that also thyroglobulin antibodies in patients sera can be demonstrated using the defined antigen substrate spheres (DASS) system with thyroglobulin as antigen. Sera of 45 patients with various thyroid diseases were investigated for the presence of thyroglobulin antibodies using passive haemagglutination and the quantitative immunofluorescence technique. Comparable results were obtained with both techniques. Advantages and disadvantages of both methods are discussed.