油菜籽甲酯和氢化植物油燃烧废气在肺上皮(A549)细胞中的遗传毒性。

IF 2.5 4区 医学 Q3 GENETICS & HEREDITY
Mutagenesis Pub Date : 2023-08-24 DOI:10.1093/mutage/gead016
Monika Hezareh Rothmann, Peter Møller, Yona J Essig, Louise Gren, Vilhelm B Malmborg, Martin Tunér, Joakim Pagels, Annette M Krais, Martin Roursgaard
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引用次数: 1

摘要

生物燃料是石油柴油的一种有吸引力的替代品,因为它对环境的影响较小。例如,油菜籽甲酯每燃料能量含量的多环芳烃排放量低于石油柴油。本研究通过石油柴油、RME和氢化植物油(HVO)在肺上皮(A549)细胞中燃烧产生的废气颗粒的可提取有机物(EOM)来评估遗传毒性。基因毒性通过碱性彗星试验评估为DNA链断裂。基于相同浓度的总PAH,来自石油柴油燃烧的EOM和RME产生相同水平的DNA链断裂(即每百万个碱基对分别增加0.13[95%置信区间(CI):00.002、0.25和0.12[95%可信区间:0.01、0.24]个损伤)。相比之下,阳性对照(依托泊苷)每百万个碱基对产生更高水平的DNA链断裂(即0.84,95%CI:0.72,0.97)损伤。RME和HVO燃烧颗粒产生的EOM浓度相对较低(
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genotoxicity by rapeseed methyl ester and hydrogenated vegetable oil combustion exhaust products in lung epithelial (A549) cells.

Biofuel is an attractive substitute for petrodiesel because of its lower environmental footprint. For instance, the polycyclic aromatic hydrocarbons (PAH) emission per fuel energy content is lower for rapeseed methyl ester (RME) than for petrodiesel. This study assesses genotoxicity by extractable organic matter (EOM) of exhaust particles from the combustion of petrodiesel, RME, and hydrogenated vegetable oil (HVO) in lung epithelial (A549) cells. Genotoxicity was assessed as DNA strand breaks by the alkaline comet assay. EOM from the combustion of petrodiesel and RME generated the same level of DNA strand breaks based on the equal concentration of total PAH (i.e. net increases of 0.13 [95% confidence interval (CI): 0.002, 0.25, and 0.12 [95% CI: 0.01, 0.24] lesions per million base pairs, respectively). In comparison, the positive control (etoposide) generated a much higher level of DNA strand breaks (i.e. 0.84, 95% CI: 0.72, 0.97) lesions per million base pairs. Relatively low concentrations of EOM from RME and HVO combustion particles (<116 ng/ml total PAH) did not cause DNA strand breaks in A549 cells, whereas benzo[a]pyrene and PAH-rich EOM from petrodiesel combusted using low oxygen inlet concentration were genotoxic. The genotoxicity was attributed to high molecular weight PAH isomers with 5-6 rings. In summary, the results show that EOM from the combustion of petrodiesel and RME generate the same level of DNA strand breaks on an equal total PAH basis. However, the genotoxic hazard of engine exhaust from on-road vehicles is lower for RME than petrodiesel because of lower PAH emission per fuel energy content.

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来源期刊
Mutagenesis
Mutagenesis 生物-毒理学
CiteScore
5.90
自引率
3.70%
发文量
22
审稿时长
6-12 weeks
期刊介绍: Mutagenesis is an international multi-disciplinary journal designed to bring together research aimed at the identification, characterization and elucidation of the mechanisms of action of physical, chemical and biological agents capable of producing genetic change in living organisms and the study of the consequences of such changes.
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