成年小鼠离体脑切片穿孔膜片钳记录。

Simon Hess, Helmut Wratil, Peter Kloppenburg
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引用次数: 0

摘要

细胞内信号通路直接或间接调节神经元活动。在使用尖锐电极或全细胞膜片钳记录的细胞电生理测量中,这些信号通路被干扰的风险很大,显著改变了被测神经元的电生理特性。穿孔膜片钳记录避免了这个问题,允许长期电生理记录,最小化细胞内环境的损害。基于先前的研究,我们描述了一种无迷信的方案,可用于常规执行穿孔膜片钳记录电流和电压测量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Perforated Patch Clamp Recordings in ex vivo Brain Slices from Adult Mice.

Perforated Patch Clamp Recordings in ex vivo Brain Slices from Adult Mice.

Perforated Patch Clamp Recordings in ex vivo Brain Slices from Adult Mice.

Perforated Patch Clamp Recordings in ex vivo Brain Slices from Adult Mice.

Intracellular signaling pathways directly and indirectly regulate neuronal activity. In cellular electrophysiological measurements with sharp electrodes or whole-cell patch clamp recordings, there is a great risk that these signaling pathways are disturbed, significantly altering the electrophysiological properties of the measured neurons. Perforated-patch clamp recordings circumvent this issue, allowing long-term electrophysiological recordings with minimized impairment of the intracellular milieu. Based on previous studies, we describe a superstition-free protocol that can be used to routinely perform perforated patch clamp recordings for current and voltage measurements.

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