Miroslav Mišík, Michael Kundi, Nadine Worel, Franziska Ferk, Hans-Peter Hutter, Michael Grusch, Armen Nersesyan, Denise Herrera Morales, Siegfried Knasmueller
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Pooled peripheral blood mononuclear cells (PBMC) from three groups [young normal weight, young obese (YO), and older age normal weight individuals] were exposed to different doses of HF-EMF (0.25, 0.5, and 1.0 W/kg specific absorption rate-SAR) and simultaneously or sequentially to different chemicals which cause DNA damage (CrO3, NiCl2, benzo[a]pyrene diol epoxide-BPDE, and 4-nitroquinoline 1-oxide-4NQO) via different molecular mechanisms. We found no difference in regard to the background values in the three groups but a significant increase of DNA damage (81% without and 36% with serum) in cells from old participants after radiation with 1.0 W/kg SAR 16 h. In combined treatment experiments we found no impact of the UMTS signal on chemically induced DNA damage in the different groups in general. However, a moderate decrease of DNA damage was seen in simultaneous treatment experiments with BPDE and 1.0 W/kg SAR in the YO group (decline 18%). Taken together our findings indicate that HF-EMF cause DNA damage in PBMC from older subjects (69.1 years). 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引用次数: 0
摘要
本研究的目的是调查人类的年龄和体重是否对高频移动电话特定电磁场(HF-EMF,1950 MHz,通用移动通信系统,UMTS信号)的DNA损伤特性有影响,以及这种形式的辐射是否对职业相关暴露的遗传毒性影响有影响。来自三组[年轻正常体重、年轻肥胖(YO)和老年正常体重个体]的汇集的外周血单核细胞(PBMC)暴露于不同剂量的HF-EMF(0.25、0.5和1.0W/kg比吸收率SAR),并同时或依次暴露于引起DNA损伤的不同化学物质(CrO3、NiCl2、苯并[a]芘二醇环氧化物BPDE和4-硝基喹啉1-氧化物4NQO)。我们发现三组的背景值没有差异,但在用1.0W/kg SAR 16小时辐射后,老年参与者的细胞中的DNA损伤显著增加(没有血清时为81%,有血清时为36%)。在联合治疗实验中,我们发现UMTS信号对不同组的化学诱导的DNA损伤没有影响。然而,在使用BPDE和1.0W/kg SAR的同时治疗实验中,YO组的DNA损伤适度减少(下降18%)。总之,我们的研究结果表明,HF-EMF会导致老年受试者(69.1岁)PBMC的DNA损伤。此外,他们还表明,辐射不会增加职业相关化学物质对DNA损伤的诱导作用。
Impact of mobile phone-specific electromagnetic fields on DNA damage caused by occupationally relevant exposures: results of ex vivo experiments with peripheral blood mononuclear cells from different demographic groups.
The aim of this study was to investigate if age and body mass of humans have an impact on the DNA-damaging properties of high-frequency mobile phone-specific electromagnetic fields (HF-EMF, 1950 MHz, universal mobile telecommunications system, UMTS signal) and if this form of radiation has an impact on the genotoxic effects of occupationally relevant exposures. Pooled peripheral blood mononuclear cells (PBMC) from three groups [young normal weight, young obese (YO), and older age normal weight individuals] were exposed to different doses of HF-EMF (0.25, 0.5, and 1.0 W/kg specific absorption rate-SAR) and simultaneously or sequentially to different chemicals which cause DNA damage (CrO3, NiCl2, benzo[a]pyrene diol epoxide-BPDE, and 4-nitroquinoline 1-oxide-4NQO) via different molecular mechanisms. We found no difference in regard to the background values in the three groups but a significant increase of DNA damage (81% without and 36% with serum) in cells from old participants after radiation with 1.0 W/kg SAR 16 h. In combined treatment experiments we found no impact of the UMTS signal on chemically induced DNA damage in the different groups in general. However, a moderate decrease of DNA damage was seen in simultaneous treatment experiments with BPDE and 1.0 W/kg SAR in the YO group (decline 18%). Taken together our findings indicate that HF-EMF cause DNA damage in PBMC from older subjects (69.1 years). Furthermore, they show that the radiation does not increase induction of DNA damage by occupationally relevant chemicals.
期刊介绍:
Mutagenesis is an international multi-disciplinary journal designed to bring together research aimed at the identification, characterization and elucidation of the mechanisms of action of physical, chemical and biological agents capable of producing genetic change in living organisms and the study of the consequences of such changes.