果蝇对粪肠球菌的免疫启动依赖于免疫耐受而不是抵抗。

IF 6.7 1区 医学 Q1 Immunology and Microbiology
PLoS Pathogens Pub Date : 2023-08-11 eCollection Date: 2023-08-01 DOI:10.1371/journal.ppat.1011567
Kevin Cabrera, Duncan S Hoard, Olivia Gibson, Daniel I Martinez, Zeba Wunderlich
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引用次数: 1

摘要

先天免疫引发增加了生物体在初次非致命感染后第二次感染的存活率。我们使用果蝇和一种昆虫来源的粪肠球菌菌株来研究启动的转录控制。与其他病原体相比,引发动物存活率的提高与粪便大肠杆菌载量的减少无关。进一步的分析表明,引发的生物体能够耐受而不是抵抗感染。使用免疫组织的RNA-seq,我们发现许多基因仅在启动的苍蝇中上调,这表明对初始和继发感染有不同的转录程序。相反,很少有基因在整个实验中持续表达或在再次感染时更有效地重新激活。对免疫缺陷突变体的启动实验表明,Imd在很大程度上对单一感染的反应是可有可无的,但需要完全启动。总之,这表明苍蝇的先天免疫反应在免疫策略、转录程序和途径使用方面存在可塑性差异,这取决于感染史。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Drosophila immune priming to Enterococcus faecalis relies on immune tolerance rather than resistance.

Drosophila immune priming to Enterococcus faecalis relies on immune tolerance rather than resistance.

Drosophila immune priming to Enterococcus faecalis relies on immune tolerance rather than resistance.

Drosophila immune priming to Enterococcus faecalis relies on immune tolerance rather than resistance.

Innate immune priming increases an organism's survival of a second infection after an initial, non-lethal infection. We used Drosophila melanogaster and an insect-derived strain of Enterococcus faecalis to study transcriptional control of priming. In contrast to other pathogens, the enhanced survival in primed animals does not correlate with decreased E. faecalis load. Further analysis shows that primed organisms tolerate, rather than resist infection. Using RNA-seq of immune tissues, we found many genes were upregulated in only primed flies, suggesting a distinct transcriptional program in response to initial and secondary infections. In contrast, few genes continuously express throughout the experiment or more efficiently re-activate upon reinfection. Priming experiments in immune deficient mutants revealed Imd is largely dispensable for responding to a single infection but needed to fully prime. Together, this indicates the fly's innate immune response is plastic-differing in immune strategy, transcriptional program, and pathway use depending on infection history.

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来源期刊
PLoS Pathogens
PLoS Pathogens 生物-病毒学
CiteScore
11.40
自引率
3.00%
发文量
598
审稿时长
2 months
期刊介绍: Bacteria, fungi, parasites, prions and viruses cause a plethora of diseases that have important medical, agricultural, and economic consequences. Moreover, the study of microbes continues to provide novel insights into such fundamental processes as the molecular basis of cellular and organismal function.
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