Abbas Heydari, Sinan Anlaş, Hasan Bakhshi, Mona Koosha, Nayyereh Choubdar, Somayeh Panahi-Moghadam, Mohammad Ali Oshaghi
{"title":"伊朗北部里海沿岸人类线状皮炎病原拟蚊属(鞘翅目:葡萄球菌科，拟蚊科)的分子特征。","authors":"Abbas Heydari, Sinan Anlaş, Hasan Bakhshi, Mona Koosha, Nayyereh Choubdar, Somayeh Panahi-Moghadam, Mohammad Ali Oshaghi","doi":"10.18502/jad.v17i1.13206","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>A combined morphological and molecular survey was performed to determine the agent of human linear dermatitis <i>Paederus</i> Fabricius, 1775 (Coleoptera: Staphylinidae, Paederinae) species composition in Mazandaran Province in the Caspian Sea coast in northern Iran, where most of linear dermatitis cases of the country occurred.</p><p><strong>Methods: </strong>Altogether, 397 <i>Paederus</i> specimens were collected from May to August 2021 and classified using morphological characters and ITS2-rDNA sequence analysis.</p><p><strong>Results: </strong>Morphological investigation revealed that all the specimens were <i>Paederus fuscipes.</i> ITS2 polymerase chain reaction (PCR) direct-sequences and the profiles of restriction fragment length polymorphism (RFLP) derived from digestion of PCR products by <i>Hinf</i>I, <i>Hpa</i>II, and <i>Sal</i>I enzymes were identical confirming the morphological results, implying that all specimens belonged to a single taxon.</p><p><strong>Conclusion: </strong><i>Paederus fuscipes</i> (Fabricius, 1775) is considered the dominant taxon and responsible for linear dermatitis in Mazandaran Province. To our knowledge, we have provided the first molecular typing of <i>Paederus</i> beetles at the species level, suggesting that ITS2-rDNA characterization is an alternative tool for species discrimination of <i>Paederus</i> spp.</p>","PeriodicalId":15095,"journal":{"name":"Journal of Arthropod-Borne Diseases","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/19/51/JAD-17-94.PMC10440500.pdf","citationCount":"0","resultStr":"{\"title\":\"Molecular Characterization of <i>Paederus</i> Spp (Coleoptera: Staphylinidae, Paederinae) the Agent of Human Linear Dermatitis in the Caspian Sea Coast, North of Iran.\",\"authors\":\"Abbas Heydari, Sinan Anlaş, Hasan Bakhshi, Mona Koosha, Nayyereh Choubdar, Somayeh Panahi-Moghadam, Mohammad Ali Oshaghi\",\"doi\":\"10.18502/jad.v17i1.13206\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>A combined morphological and molecular survey was performed to determine the agent of human linear dermatitis <i>Paederus</i> Fabricius, 1775 (Coleoptera: Staphylinidae, Paederinae) species composition in Mazandaran Province in the Caspian Sea coast in northern Iran, where most of linear dermatitis cases of the country occurred.</p><p><strong>Methods: </strong>Altogether, 397 <i>Paederus</i> specimens were collected from May to August 2021 and classified using morphological characters and ITS2-rDNA sequence analysis.</p><p><strong>Results: </strong>Morphological investigation revealed that all the specimens were <i>Paederus fuscipes.</i> ITS2 polymerase chain reaction (PCR) direct-sequences and the profiles of restriction fragment length polymorphism (RFLP) derived from digestion of PCR products by <i>Hinf</i>I, <i>Hpa</i>II, and <i>Sal</i>I enzymes were identical confirming the morphological results, implying that all specimens belonged to a single taxon.</p><p><strong>Conclusion: </strong><i>Paederus fuscipes</i> (Fabricius, 1775) is considered the dominant taxon and responsible for linear dermatitis in Mazandaran Province. To our knowledge, we have provided the first molecular typing of <i>Paederus</i> beetles at the species level, suggesting that ITS2-rDNA characterization is an alternative tool for species discrimination of <i>Paederus</i> spp.</p>\",\"PeriodicalId\":15095,\"journal\":{\"name\":\"Journal of Arthropod-Borne Diseases\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2023-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/19/51/JAD-17-94.PMC10440500.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Arthropod-Borne Diseases\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.18502/jad.v17i1.13206\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Arthropod-Borne Diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.18502/jad.v17i1.13206","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Molecular Characterization of Paederus Spp (Coleoptera: Staphylinidae, Paederinae) the Agent of Human Linear Dermatitis in the Caspian Sea Coast, North of Iran.
Background: A combined morphological and molecular survey was performed to determine the agent of human linear dermatitis Paederus Fabricius, 1775 (Coleoptera: Staphylinidae, Paederinae) species composition in Mazandaran Province in the Caspian Sea coast in northern Iran, where most of linear dermatitis cases of the country occurred.
Methods: Altogether, 397 Paederus specimens were collected from May to August 2021 and classified using morphological characters and ITS2-rDNA sequence analysis.
Results: Morphological investigation revealed that all the specimens were Paederus fuscipes. ITS2 polymerase chain reaction (PCR) direct-sequences and the profiles of restriction fragment length polymorphism (RFLP) derived from digestion of PCR products by HinfI, HpaII, and SalI enzymes were identical confirming the morphological results, implying that all specimens belonged to a single taxon.
Conclusion: Paederus fuscipes (Fabricius, 1775) is considered the dominant taxon and responsible for linear dermatitis in Mazandaran Province. To our knowledge, we have provided the first molecular typing of Paederus beetles at the species level, suggesting that ITS2-rDNA characterization is an alternative tool for species discrimination of Paederus spp.
期刊介绍：
The journal publishes original research paper, short communication, scientific note, case report, letter to the editor, and review article in English. The scope of papers comprises all aspects of arthropod borne diseases including:
● Systematics
● Vector ecology
● Epidemiology
● Immunology
● Parasitology
● Molecular biology
● Genetics
● Population dynamics
● Toxicology
● Vector control
● Diagnosis and treatment and other related subjects.
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