cacna2d3缺陷小鼠听觉皮层群体活动和局部调谐异质性的改变。

IF 2.9 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Simon L Wadle, Tatjana T X Schmitt, Jutta Engel, Simone Kurt, Jan J Hirtz
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引用次数: 0

摘要

电压激活钙通道α2δ3辅助亚基是听觉脑干正常突触传递和声音的精确时间加工所必需的。在小鼠中,它的丧失还会导致无法分辨调幅音调。此外,α2δ3的功能丧失与人类自闭症谱系障碍有关。为了研究α2δ3基因敲除小鼠高阶听觉系统网络活动的可能变化,我们使用双光子Ca2+成像分析了听觉皮层(AC)网络内的神经元活动模式和频率调谐地形。与野生型小鼠相比,我们在初级听觉皮层中发现了明显的亚场特异性变化,表现为对不同声音做出反应的网络活动模式之间的总体相关性较低,以及这些模式在重复相同声音时的可靠性较低。较高的交流子场没有表现出这些改变,但显示出更多的调谐良好的神经元,同时神经元频率调谐的局部异质性较低。我们的结果为自闭症谱系障碍相关小鼠模型中交流网络活动的改变提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Altered population activity and local tuning heterogeneity in auditory cortex of Cacna2d3-deficient mice.

The α2δ3 auxiliary subunit of voltage-activated calcium channels is required for normal synaptic transmission and precise temporal processing of sounds in the auditory brainstem. In mice its loss additionally leads to an inability to distinguish amplitude-modulated tones. Furthermore, loss of function of α2δ3 has been associated with autism spectrum disorder in humans. To investigate possible alterations of network activity in the higher-order auditory system in α2δ3 knockout mice, we analyzed neuronal activity patterns and topography of frequency tuning within networks of the auditory cortex (AC) using two-photon Ca2+ imaging. Compared to wild-type mice we found distinct subfield-specific alterations in the primary auditory cortex, expressed in overall lower correlations between the network activity patterns in response to different sounds as well as lower reliability of these patterns upon repetitions of the same sound. Higher AC subfields did not display these alterations but showed a higher amount of well-tuned neurons along with lower local heterogeneity of the neurons' frequency tuning. Our results provide new insight into AC network activity alterations in an autism spectrum disorder-associated mouse model.

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来源期刊
Biological Chemistry
Biological Chemistry 生物-生化与分子生物学
CiteScore
7.20
自引率
0.00%
发文量
63
审稿时长
4-8 weeks
期刊介绍: Biological Chemistry keeps you up-to-date with all new developments in the molecular life sciences. In addition to original research reports, authoritative reviews written by leading researchers in the field keep you informed about the latest advances in the molecular life sciences. Rapid, yet rigorous reviewing ensures fast access to recent research results of exceptional significance in the biological sciences. Papers are published in a "Just Accepted" format within approx.72 hours of acceptance.
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