猪刚地弓形虫特异性抗体的检测:优点与局限性

IF 3.7 2区 医学 Q1 PARASITOLOGY
Johanna Kauter , Filip Damek , Gereon Schares , Radu Blaga , Franziska Schott , Peter Deplazes , Xaver Sidler , Walter Basso
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引用次数: 1

摘要

弓形虫是一种主要的食源性寄生虫,感染猪的未煮熟的肉是人类感染的重要来源。由于猪的感染大多是亚临床的,因此需要进行足够的诊断测试,以便在农场层面使用。口服液(OF)被证明是一种很有前途的直接和间接检测猪各种病原体感染的基质。本研究的目的是评估是否可以使用适用于of样本的间接ELISA试剂盒(of-ELISA)诊断猪弓形虫感染。常规血清学和OF免疫印迹(IB)作为比较的标准。为此,通过ELISA分析了来自实验性接种弓形虫卵囊的母猪(n=8)和育肥者(n=3)的系列OF样本、来自潜在暴露母猪(n=9)的个体田间样本和来自潜在暴露组饲养的育肥者的合并OF样本(n=195个猪组,包括2248只动物)的抗弓形虫抗体。对于个体动物,OF-ELISA表现出97.3%的相对诊断特异性和78.8%的相对诊断敏感性。在实验感染的动物中,从接种后1.5周(pi)到实验设置结束(8至30周pi)观察到阳性OF-ELISA结果;然而,尽管血清阳性率持续,但在一些动物中偶尔会观察到低于估计临界值的值。在潜在暴露的个体动物中,OF和血清ELISA结果显示100%一致。在饲养肥猪的群体中,OF-ELISA可以在至少25%的动物血清呈阳性的群体中可靠地检测到针对弓形虫的抗体。这种基于市售血清ELISA的OF-ELISA可能是一种有趣的非侵入性筛查工具,用于检测农场水平上高暴露于弓形虫的猪群。OF-ELISA可能需要进一步调整,以一致地检测个体感染猪,这可能是由于OF抗体浓度随时间的变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Detection of Toxoplasma gondii-specific antibodies in pigs using an oral fluid-based commercial ELISA: Advantages and limitations

Detection of Toxoplasma gondii-specific antibodies in pigs using an oral fluid-based commercial ELISA: Advantages and limitations

Toxoplasma gondii is a major food-borne parasite and undercooked meat of infected pigs represents an important source of infection for humans. Since infections in pigs are mostly subclinical, adequate diagnostic tests for use at the farm level are pursued. Oral fluid (OF) was shown to be a promising matrix for direct and indirect detection of infections with various pathogens in pigs. The objective of this study was to assess whether T. gondii infections in pigs could be diagnosed using an indirect ELISA kit adapted for OF samples (OF-ELISA). Routine serology and OF-immunoblot (IB) were used as standards for the comparison. For this, serial OF samples from sows (n = 8) and fatteners (n = 3) experimentally inoculated with T. gondii oocysts, individual field samples from potentially exposed sows (n = 9) and pooled OF samples from potentially exposed group-housed fatteners (n = 195 pig groups, including 2,248 animals) were analysed for antibodies against T. gondii by ELISA. For individual animals, OF-ELISA exhibited a relative diagnostic specificity of 97.3% and a relative diagnostic sensitivity of 78.8%. In experimentally infected animals, positive OF-ELISA results were observed from 1.5 weeks post inoculation (pi) until the end of the experimental setup (8 to 30 weeks pi); however, values below the estimated cut-off were occasionally observed in some animals despite constant seropositivity. In potentially exposed individual animals, OF- and serum-ELISA results showed 100% agreement. In group-housed fatteners, antibodies against T. gondii could be reliably detected by OF-ELISA in groups in which at least 25% of the animals were seropositive. This OF-ELISA, based on a commercially available serum-ELISA, may represent an interesting non-invasive screening tool for detecting pig groups with a high exposure to T. gondii at the farm level. The OF-ELISA may need further adjustments to consistently detect individual infected pigs, probably due to variations in OF antibody concentration over time.

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来源期刊
CiteScore
8.40
自引率
2.50%
发文量
76
审稿时长
23 days
期刊介绍: International Journal for Parasitology offers authors the option to sponsor nonsubscriber access to their articles on Elsevier electronic publishing platforms. For more information please view our Sponsored Articles page. The International Journal for Parasitology publishes the results of original research in all aspects of basic and applied parasitology, including all the fields covered by its Specialist Editors, and ranging from parasites and host-parasite relationships of intrinsic biological interest to those of social and economic importance in human and veterinary medicine and agriculture.
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