Effects of cyclophosphamide on rat placental development.

IF 0.9 4区 医学 Q4 PATHOLOGY
Satoshi Furukawa, Naho Tsuji, Seigo Hayashi, Yusuke Kuroda, Masayuki Kimura, Chisato Kojima, Kazuya Takeuchi
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引用次数: 0

Abstract

We examined the morphological effects of cyclophosphamide (CPA) on placental development in pregnant rats. CPA was administered as a single dose to pregnant rats intraperitoneally at 0 mg/kg (the control group), 25 mg/kg on gestation day (GD) 12 (the CPA GD 12-treated group), and 25 mg/kg on GD 14 (the CPA GD 14-treated group). The fetal and placental weight decreased in the CPA-treated groups, complete fetal resorption from GD 17 onwards in the CPA GD 12-treated group, and external malformations in the CPA GD 14-treated group. Histopathologically, CPA induced apoptosis and/or cell proliferation inhibition in each part of the placenta. In the labyrinth zone, syncytiotrophoblasts were selectively reduced, resulting in a small placenta. In the basal zone, the number of spongiotrophoblasts was reduced, resulting in hypoplasia of glycogen cell islands. In addition, a small number of interstitial trophoblasts invaded the metrial gland from the basal zone on GD 15. The severity of these lesions was higher in the CPA GD 12-treated group than in the CPA GD 14-treated group. In the metrial gland, although the number of uterine natural killer cells was reduced, metrial gland development was not affected.

环磷酰胺对大鼠胎盘发育的影响。
我们研究了环磷酰胺(CPA)对妊娠大鼠胎盘发育的形态学影响。妊娠大鼠单次腹腔注射CPA,剂量为0 mg/kg(对照组),妊娠第12天注射25 mg/kg (CPA GD 12治疗组),妊娠第14天注射25 mg/kg (CPA GD 14治疗组)。cppa处理组胎儿和胎盘重量下降,cpdp 12处理组胎儿从gdp 17开始完全吸收,cpdp 14处理组胎儿出现外部畸形。在组织病理学上,CPA诱导胎盘各部分细胞凋亡和/或细胞增殖抑制。在迷宫区,合胞滋养细胞选择性减少,形成小胎盘。在基底区,海绵滋养细胞数量减少,导致糖原细胞岛发育不全。此外,在GD 15时,少量间质滋养细胞从基区向子宫腺侵袭。这些病变的严重程度在CPA GD 12治疗组高于CPA GD 14治疗组。在子宫内膜腺中,子宫自然杀伤细胞数量减少,但不影响子宫内膜腺的发育。
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来源期刊
Journal of Toxicologic Pathology
Journal of Toxicologic Pathology PATHOLOGY-TOXICOLOGY
CiteScore
2.10
自引率
16.70%
发文量
22
审稿时长
>12 weeks
期刊介绍: JTP is a scientific journal that publishes original studies in the field of toxicological pathology and in a wide variety of other related fields. The main scope of the journal is listed below. Administrative Opinions of Policymakers and Regulatory Agencies Adverse Events Carcinogenesis Data of A Predominantly Negative Nature Drug-Induced Hematologic Toxicity Embryological Pathology High Throughput Pathology Historical Data of Experimental Animals Immunohistochemical Analysis Molecular Pathology Nomenclature of Lesions Non-mammal Toxicity Study Result or Lesion Induced by Chemicals of Which Names Hidden on Account of the Authors Technology and Methodology Related to Toxicological Pathology Tumor Pathology; Neoplasia and Hyperplasia Ultrastructural Analysis Use of Animal Models.
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