Investigation of the association of the RAN (rs14035) and XPO5 (rs11077) polymorphisms with venous thromboembolism.

IF 1.6 Q2 MEDICINE, GENERAL & INTERNAL

Romanian Journal of Internal Medicine Pub Date : 2023-09-01 DOI:10.2478/rjim-2023-0014

Khloud M Alquraan, Omar F Khabour

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引用次数: 0

Abstract

Introduction: Venous thromboembolism (VTE) is the third most common hemostatic disease worldwide. Studies have reported a role for microRNA (miRNA) in the homeostasis and development of VTE. The ras-related nuclear protein (RAN) and exportin 5 (XPO5) genes are involved in miRNA biogenesis, as both regulate the transport of pre-miRNA from the nucleus to the cytoplasm. Therefore, the aim of the current study is to examine the association between RAN (rs14035) and XPO5 (rs11077) single nucleotide polymorphisms (SNPs) and VTE.

Methods: The study sample consisted of 300 subjects (150 patients and 150 age and sex matched controls). The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and tetra-primer amplification refractory mutation system (T-ARMS) techniques were used to genotype rs14035 and rs11077, respectively.

Results: The results showed that there was a significant association between the XPO5 rs11077 and the risk of VTE (P < 0.05). Subjects with AC (OR: 2.08, CI:1.26-3.44) and CC (OR: 1.77, CI: 0.88-3.55) genotypes were at increased risk of the developing VTE. Regarding RAN gene, no association was found between rs14035 and VTE (P > 0.05). In addition, no associations were found between XPO5 rs11077 and RAN rs14035 genotypes with blood cell parameters (P > 0.05). As for the demographic characteristics, the results indicated a strong association between family history and body mass index (BMI) with the risk of VTE (P < 0.01).

Conclusion: The XPO5 rs11077, BMI and family history might contribute to the development of VTE in Jordan.

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RAN (rs14035)和XPO5 (rs11077)多态性与静脉血栓栓塞的相关性研究

简介:静脉血栓栓塞(VTE)是世界上第三大最常见的止血疾病。研究报道了microRNA (miRNA)在静脉血栓栓塞的动态平衡和发展中的作用。ras相关核蛋白(RAN)和输出蛋白5 (XPO5)基因参与miRNA的生物发生，它们都调节miRNA前体从细胞核到细胞质的运输。因此，本研究的目的是研究RAN (rs14035)和XPO5 (rs11077)单核苷酸多态性(snp)与VTE之间的关系。方法:研究样本包括300名受试者(150名患者和150名年龄和性别匹配的对照组)。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)和四引物扩增难突变系统(T-ARMS)技术分别对rs14035和rs11077进行基因分型。结果:XPO5 rs11077与VTE发生风险有显著相关性(P < 0.05)。AC基因型(OR: 2.08, CI:1.26-3.44)和CC基因型(OR: 1.77, CI: 0.88-3.55)的受试者发生静脉血栓栓塞的风险增加。对于RAN基因，rs14035与VTE无相关性(P > 0.05)。此外，XPO5 rs11077和RAN rs14035基因型与血细胞参数无相关性(P > 0.05)。在人口学特征方面，家族史和体重指数(BMI)与血栓栓塞风险有较强的相关性(P < 0.01)。结论:XPO5 rs11077、BMI和家族史可能与约旦VTE的发生有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Romanian Journal of Internal Medicine MEDICINE, GENERAL & INTERNAL-

CiteScore

3.20

自引率

5.30%

发文量

审稿时长

15 weeks

期刊介绍： Romanian Journal of Physics is a journal publishing physics contributions on the following themes: •Theoretical Physics & Applied Mathematics •Nuclear Physics •Solid State Physics & Materials Science •Statistical Physics & Quantum Mechanics •Optics •Spectroscopy •Plasma & Lasers •Nuclear & Elementary Particles Physics •Atomic and Molecular Physics •Astrophysics •Atmosphere and Earth Science •Environment Protection