PRSS2 regulates EMT and metastasis via MMP-9 in gastric cancer

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2023-08-01 DOI:10.1016/j.acthis.2023.152071

Fei Wang , Jianfeng Yi , Yu Chen , Xiang Bai , Chunfeng Lu , Shichun Feng , Xiaojun Zhou

{"title":"PRSS2 regulates EMT and metastasis via MMP-9 in gastric cancer","authors":"Fei Wang , Jianfeng Yi , Yu Chen , Xiang Bai , Chunfeng Lu , Shichun Feng , Xiaojun Zhou","doi":"10.1016/j.acthis.2023.152071","DOIUrl":null,"url":null,"abstract":"<div>Serine protease 2 (PRSS2) is upregulated in gastric cancer tissues, correlates with poor prognosis and promotes migration and invasion of gastric cancer cells. However, the exact mechanism by which PRSS2 promotes metastasis in gastric cancer is unclear. We examined serum PRSS2 levels in healthy controls and gastric cancer patients by enzyme linked immunosorbent assay (ELISA) and analyzed the correlation between PRSS2 serum level with the clinicopathological characteristics of gastric cancer patients and matrix metalloproteinase-9 (MMP-9) expression. A lentiviral MMP-9 overexpression vector was constructed and used to transfect gastric cancer cells with stable silencing of PRSS2, and migration, invasion and epithelial-mesenchymal transition (EMT) of gastric cancer cells were examined. High serum PRSS2 levels were detected in gastric cancer patients and associated with lymphatic metastasis and TNM stage. Serum PRSS2 was positively correlated with serum MMP-9 level. PRSS2 silencing inhibited EMT, and knock-down of PRSS2 partially abrogated cell metastasis and EMT caused by overexpression of MMP-9. These results suggest that PRSS2 promotes the migration and invasion of gastric cancer cells through EMT induction by MMP-9. Our findings suggest that PRSS2 may be a potential early diagnostic marker and therapeutic target of gastric cancer.</div>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0065128123000776","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}

引用次数: 1

Abstract

Serine protease 2 (PRSS2) is upregulated in gastric cancer tissues, correlates with poor prognosis and promotes migration and invasion of gastric cancer cells. However, the exact mechanism by which PRSS2 promotes metastasis in gastric cancer is unclear. We examined serum PRSS2 levels in healthy controls and gastric cancer patients by enzyme linked immunosorbent assay (ELISA) and analyzed the correlation between PRSS2 serum level with the clinicopathological characteristics of gastric cancer patients and matrix metalloproteinase-9 (MMP-9) expression. A lentiviral MMP-9 overexpression vector was constructed and used to transfect gastric cancer cells with stable silencing of PRSS2, and migration, invasion and epithelial-mesenchymal transition (EMT) of gastric cancer cells were examined. High serum PRSS2 levels were detected in gastric cancer patients and associated with lymphatic metastasis and TNM stage. Serum PRSS2 was positively correlated with serum MMP-9 level. PRSS2 silencing inhibited EMT, and knock-down of PRSS2 partially abrogated cell metastasis and EMT caused by overexpression of MMP-9. These results suggest that PRSS2 promotes the migration and invasion of gastric cancer cells through EMT induction by MMP-9. Our findings suggest that PRSS2 may be a potential early diagnostic marker and therapeutic target of gastric cancer.

查看原文本刊更多论文

PRSS2通过MMP-9调控胃癌的EMT和转移

丝氨酸蛋白酶2（PRSS2）在癌症组织中上调，与预后不良相关，并促进癌症细胞的迁移和侵袭。然而，PRSS2促进癌症转移的确切机制尚不清楚。采用酶联免疫吸附试验（ELISA）检测了健康对照组和癌症患者血清PRSS2水平，并分析了PRSS2与癌症患者临床病理特征和基质金属蛋白酶-9（MMP-9）表达的相关性。构建慢病毒MMP-9过表达载体，转染稳定沉默PRSS2的癌症细胞，检测癌症细胞的迁移、侵袭和上皮-间质转移（EMT）。癌症患者血清PRSS2水平升高，与淋巴结转移和TNM分期有关。血清PRSS2与血清MMP-9水平呈正相关。PRSS2沉默抑制EMT，并且PRSS2的敲除部分消除了由MMP-9过表达引起的细胞转移和EMT。这些结果表明，PRSS2通过MMP-9的EMT诱导促进癌症细胞的迁移和侵袭。我们的研究结果表明，PRSS2可能是癌症潜在的早期诊断标志物和治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464