Study of platelet-rich fibrin promoting endothelial cell differentiation and angiogenesis induced by transplantation of adipose-derived stem cells

IF 2.3 4区生物学 Q4 CELL BIOLOGY

Acta histochemica Pub Date : 2023-08-01 DOI:10.1016/j.acthis.2023.152059

Zhibing Ma , Jin Ding , Yawen Wang , Tianqi Zhang , Gang Chen , Jinlong Huang

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引用次数: 0

Abstract

Diabetic patients are characterized by long wound healing time, and adipose stem cells (ADSCs) can secrete growth factors to promote angiogenesis and improve diabetic wound healing. In this research, we attempted to interrogate the impact of platelet-rich fibrin (PRF) on ADSCs in diabetic wound healing. ADSCs were harvested from human adipose tissues and identified through flow cytometry. After pretreatment with cultured medium supplemented with different concentrations of PRF (2.5%, 5%, and 7.5%), proliferation and differentiation capacity of ADSCs were assessed by CCK-8 assay, qRT-PCR and immunofluorescence (IF), respectively. Tube formation assay measured angiogenesis. Western blot analysis analyzed expression of endothelial markers and the extracellular signal-regulated kinase (ERK) and serine/threonine kinase (Akt) pathways in PRF-induced ADSCs. The CCK-8 experiment indicated that PRF enhanced proliferation of ADSCs in dose-dependent manner, relative to normal control group. The expression of endothelial markers and the capacity of tube formation were significantly promoted by 7.5% PRF. The release of growth factors containing vascular endothelial grow factor (VEGF) and insulin-like growth factor-1 (IGF-1) from PRF was increased with the extension of detection time. When the receptors of VEGF or/and IGF-1 were neutralized, ADSCs differentiation into endothelial cells were obviously inhibited. Additionally, PRF stimulated ERK and Akt pathways, and the inhibitors of ERK and Akt attenuated PRF-induced differentiation of ADSCs into endothelial cells. In conclusion, PRF promoted endothelial cell differentiation and angiogenesis induced by ADSCs in diabetic wound healing, which appears to give guidance for treating patients.

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富血小板纤维蛋白促进脂肪干细胞移植诱导内皮细胞分化和血管生成的研究

糖尿病患者的特点是伤口愈合时间长，脂肪干细胞（ADSCs）可以分泌生长因子促进血管生成，改善糖尿病伤口愈合。在这项研究中，我们试图探讨富含血小板的纤维蛋白（PRF）在糖尿病伤口愈合中对ADSCs的影响。从人类脂肪组织中采集ADSCs，并通过流式细胞术进行鉴定。用添加不同浓度PRF（2.5%、5%和7.5%）的培养基预处理后，分别通过CCK-8测定、qRT-PCR和免疫荧光（IF）评估ADSCs的增殖和分化能力。试管形成测定法测量血管生成。Western印迹分析分析了内皮标记物和细胞外信号调节激酶（ERK）和丝氨酸/苏氨酸激酶（Akt）途径在PRF诱导的ADSCs中的表达。CCK-8实验表明，与正常对照组相比，PRF以剂量依赖的方式增强ADSCs的增殖。7.5%的PRF显著促进了内皮标志物的表达和管形成能力。PRF中含有血管内皮生长因子（VEGF）和胰岛素样生长因子-1（IGF-1）的生长因子的释放随着检测时间的延长而增加。当VEGF或/和IGF-1受体被中和时，ADSCs向内皮细胞的分化受到明显抑制。此外，PRF刺激ERK和Akt途径，ERK和Akt抑制剂减弱了PRF诱导的ADSCs向内皮细胞的分化。总之，在糖尿病伤口愈合中，PRF促进了ADSCs诱导的内皮细胞分化和血管生成，这似乎为治疗患者提供了指导。

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来源期刊

Acta histochemica 生物-细胞生物学

CiteScore

4.60

自引率

4.00%

发文量

107

审稿时长

23 days

期刊介绍： Acta histochemica, a journal of structural biochemistry of cells and tissues, publishes original research articles, short communications, reviews, letters to the editor, meeting reports and abstracts of meetings. The aim of the journal is to provide a forum for the cytochemical and histochemical research community in the life sciences, including cell biology, biotechnology, neurobiology, immunobiology, pathology, pharmacology, botany, zoology and environmental and toxicological research. The journal focuses on new developments in cytochemistry and histochemistry and their applications. Manuscripts reporting on studies of living cells and tissues are particularly welcome. Understanding the complexity of cells and tissues, i.e. their biocomplexity and biodiversity, is a major goal of the journal and reports on this topic are especially encouraged. Original research articles, short communications and reviews that report on new developments in cytochemistry and histochemistry are welcomed, especially when molecular biology is combined with the use of advanced microscopical techniques including image analysis and cytometry. Letters to the editor should comment or interpret previously published articles in the journal to trigger scientific discussions. Meeting reports are considered to be very important publications in the journal because they are excellent opportunities to present state-of-the-art overviews of fields in research where the developments are fast and hard to follow. Authors of meeting reports should consult the editors before writing a report. The editorial policy of the editors and the editorial board is rapid publication. Once a manuscript is received by one of the editors, an editorial decision about acceptance, revision or rejection will be taken within a month. It is the aim of the publishers to have a manuscript published within three months after the manuscript has been accepted