Ludovic Autin, Brett A Barbaro, Andrew I Jewett, Axel Ekman, Shruti Verma, Arthur J Olson, David S Goodsell
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引用次数: 0
Abstract
Models of insulin secretory vesicles from pancreatic beta cells have been created using the cellPACK suite of tools to research, curate, construct and visualise the current state of knowledge. The model integrates experimental information from proteomics, structural biology, cryoelectron microscopy and X-ray tomography, and is used to generate models of mature and immature vesicles. A new method was developed to generate a confidence score that reconciles inconsistencies between three available proteomes using expert annotations of cellular localisation. The models are used to simulate soft X-ray tomograms, allowing quantification of features that are observed in experimental tomograms, and in turn, allowing interpretation of X-ray tomograms at the molecular level.
胰岛β细胞胰岛素分泌泡模型是利用 cellPACK 工具套件创建的，用于研究、整理、构建和可视化当前的知识状况。该模型整合了蛋白质组学、结构生物学、冷冻电镜和 X 射线断层扫描的实验信息，用于生成成熟和未成熟囊泡的模型。开发了一种新方法来生成置信度分数，利用专家对细胞定位的注释来调和三个可用蛋白质组之间的不一致性。这些模型用于模拟软 X 射线层析成像，可以量化在实验层析成像中观察到的特征，进而在分子水平上解释 X 射线层析成像。
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