Live Imaging of the Lung
Q1 Health Professions
Tomasz Brzoska, Tomasz W. Kaminski, Margaret F. Bennewitz, Prithu Sundd
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Abstract
Live imaging is critical to determining the dynamics and spatial interactions of cells within the tissue environment. In the lung, this has proven to be difficult due to the motion brought about by ventilation and cardiac contractions. A previous version of this Current Protocols in Cytometry article reported protocols for imaging ex vivo live lung slices and the intact mouse lung. Here, we update those protocols by adding new methodologies, new approaches for quantitative image analysis, and new areas of potential application. © 2020 Wiley Periodicals LLC.
Basic Protocol 1 : Live imaging of lung slices
Support Protocol 1 : Staining lung sections with fluorescent antibodies
Basic Protocol 2 : Live imaging in the mouse lung
Support Protocol 2 : Intratracheal instillations
Support Protocol 3 : Intravascular instillations
Support Protocol 4 : Monitoring vital signs of the mouse during live lung imaging
Support Protocol 5 : Antibodies
Support Protocol 6 : Fluorescent reporter mice
Basic Protocol 3 : Quantification of neutrophil-platelet aggregation in pulmonary vasculature
Basic Protocol 4 : Quantification of platelet-dependent pulmonary thrombosis
Basic Protocol 5 : Quantification of pulmonary vascular permeability
肺的实时成像
实时成像对于确定组织环境中细胞的动态和空间相互作用至关重要。在肺中，由于通气和心脏收缩带来的运动，这已被证明是困难的。这篇细胞术文章的前一个版本报道了体外活肺切片和完整小鼠肺的成像方案。在这里，我们通过添加新的方法、定量图像分析的新方法和潜在应用的新领域来更新这些协议。©2020 Wiley期刊有限公司基本方案1:肺切片的实时成像支持方案1:用荧光抗体染色肺切片基本方案2:小鼠肺的实时成像支持方案2:气管内滴注支持方案3:血管内滴注支持方案4:在活体肺成像期间监测小鼠的生命体征支持方案5:抗体支持方案6:荧光报告小鼠基本方案3:肺血管中中性粒细胞-血小板聚集的定量基本方案4:血小板依赖性肺血栓的定量基本方案5:肺血管通透性的定量
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