Bone morphogenic protein-7 (BMP-7) polymorphism: Susceptibility to cirrhosis and hepatocellular carcinoma after viral hepatitis in Egyptian patients.

IF 1.5 Q3 GASTROENTEROLOGY & HEPATOLOGY
Hind S AboShabaan, Osama Alghannam, Faisal Ismail, Islam M El-Garawani, Mohamed El-Shahat, Roba M Talaat, Eman A El-Maadawy, Nasser Hussein, Zeinab A Kasemy, Eman Abdelsameea, Soghra Haq, Heba M Hathout
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引用次数: 0

Abstract

Aim of the study: Bone morphogenic proteins (BMPs) have both inhibitory and stimulatory effects on growth of a tumor that depend on the type of cells, the dosage and the tumor microenvironment. We aimed to investigate the impact of the bone morphogenic protein-7 (BMP-7) single nucleotide polymorphism (SNP) rs230205 [A/G] on susceptibility to hepatocellular carcinoma (HCC) progression from liver cirrhosis after viral hepatitis infection in Egyptian patients.

Material and methods: The amplification-refractory mutation system (ARMS)-polymerase chain reaction (PCR) method was used to genotype the rs230205 [A/G] SNP in 150 subjects (50 patients with post-hepatitis C or B cirrhosis, 50 HCC patients, and 50 controls). Expression level of BMP-7 protein was assessed using enzyme-linked immunosorbent assay (ELISA).

Results: The results revealed insignificant changes in distribution of all genotypes/alleles of the BMP-7 rs230205 [A/G] SNP between cirrhotic patients, HCC patients and controls. The AA genotype and A allele could be considered risk factors for cirrhosis (OR = 1.75, 1.50) and HCC (OR = 2.19, 1.74), respectively. The AA genotype (95% CI: 0.45-6.79) and A allele (OR = 1.50, 95% CI: 0.77-2.93) may be viewed as cirrhosis risk factors based on group segregation. Additionally, the A allele, AG and AA genotypes and their combined ORs of 2.19 (95% CI: 0.58-8.23), 1.74 (95% CI: 0.90-3.37), and 1.70 (95% CI: 0.68-4.29) could all be risk factors for HCC. No genotype or allele could be regarded as a risk factor for progression of cirrhosis to HCC, according to OR values.

Conclusions: The results showed no correlation between BMP-7 rs230205 [A/G] SNP and progression of cirrhosis to HCC. To confirm our findings, additional prospective large-scale research is required.

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骨形态发生蛋白-7 (BMP-7)多态性:埃及患者病毒性肝炎后肝硬化和肝细胞癌的易感性
研究目的:骨形态发生蛋白(Bone morphogenic protein, BMPs)对肿瘤的生长具有抑制和促进作用,其作用取决于细胞类型、剂量和肿瘤微环境。我们旨在研究骨形态发生蛋白-7 (BMP-7)单核苷酸多态性(SNP) rs230205 [A/G]对埃及患者病毒性肝炎感染后肝硬化肝细胞癌(HCC)进展易感性的影响。材料与方法:采用扩增-难解突变系统(ARMS)-聚合酶链反应(PCR)方法对150例患者(50例丙型肝炎或乙型肝炎后肝硬化患者、50例HCC患者和50例对照组)的rs230205 [A/G] SNP进行基因分型。采用酶联免疫吸附法(ELISA)检测BMP-7蛋白表达水平。结果:结果显示BMP-7 rs230205 [A/G] SNP的所有基因型/等位基因在肝硬化患者、HCC患者和对照组之间的分布变化不显著。AA基因型和A等位基因分别是肝硬化(OR = 1.75, 1.50)和HCC (OR = 2.19, 1.74)的危险因素。AA基因型(95% CI: 0.45-6.79)和A等位基因(OR = 1.50, 95% CI: 0.77-2.93)根据组分离可视为肝硬化危险因素。此外,A等位基因、AG和AA基因型及其组合or分别为2.19 (95% CI: 0.58-8.23)、1.74 (95% CI: 0.90-3.37)和1.70 (95% CI: 0.68-4.29),都可能是HCC的危险因素。根据or值,没有基因型或等位基因可以被视为肝硬化进展为HCC的危险因素。结论:结果显示BMP-7 rs230205 [A/G] SNP与肝硬化向HCC进展无相关性。为了证实我们的发现,还需要进一步的前瞻性大规模研究。
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来源期刊
Clinical and Experimental Hepatology
Clinical and Experimental Hepatology GASTROENTEROLOGY & HEPATOLOGY-
CiteScore
2.80
自引率
0.00%
发文量
32
期刊介绍: Clinical and Experimental Hepatology – quarterly of the Polish Association for Study of Liver – is a scientific and educational, peer-reviewed journal publishing original and review papers describing clinical and basic investigations in the field of hepatology.
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