Restoration of Altered Oncogenic and Tumor Suppressor microRNA Expression in Breast Cancer and Colorectal Cancer Cell using Epicatechin.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current molecular pharmacology Pub Date : 2023-01-01 DOI:10.2174/1874467216666230210091839

Sheetal Kiran, Abhilipsa Patra, Poonam Verma, Suvendu Purkait, Gaurav Chhabra, Praveen Kumar Guttula, Amit Ghosh

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引用次数: 0

Abstract

Background: MicroRNAs (miRNA) are small non-coding RNAs that regulate the function of mRNA post-transcriptionally in a tissue-specific manner. miRNA expressions are heavily dysregulated in human cancer cells through various mechanisms, including epigenetic changes, karyotype abnormalities, and miRNA biogenesis defects. miRNAs may act as either oncogenes or tumor suppressors under different conditions. Epicatechin is a natural compound found in green tea which possesses antioxidant and antitumor properties.

Objective: The objective of this study is to investigate the effect of epicatechin treatment on the expression level of several oncogenic and tumor suppressor miRNAs in breast and colorectal cancer cell lines (MCF7 and HT-29) and identify its mechanism of action.

Methods: The MCF-7 and HT29 cells were treated with epicatechin for 24 hours and untreated cells were considered control cultures. miRNA was isolated and qRT-PCR was used to measure the expression profile changes of different oncogenic and tumor suppressor miRNAs. Furthermore, the mRNA expression profile was also screened at different concentrations of epicatechin.

Results: Our results showed several-fold changes in miRNAs expression level, which is cell line specific. Also, epicatechin at different concentrations induces biphasic changes in mRNA expression levels in both cell lines.

Conclusion: Our findings first time demonstrated that epicatechin can reverse the expression of these miRNAs and may trigger the cytostatic effect at a lower concentration.

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使用表儿茶素恢复癌症和癌症结直肠癌细胞中改变的致癌和抑癌微RNA表达。

背景：微小RNA（miRNA）是一种小的非编码RNA，以组织特异性的方式在转录后调节mRNA的功能。在人类癌症细胞中，miRNA的表达通过多种机制严重失调，包括表观遗传变化、核型异常和miRNA生物发生缺陷。miRNA在不同的条件下可以作为致癌基因或肿瘤抑制因子。表儿茶素是绿茶中的一种天然化合物，具有抗氧化和抗肿瘤的特性。目的：研究表儿茶素治疗对乳腺癌和结直肠癌癌症细胞系（MCF7和HT-29）中几种致癌和抑癌miRNA表达水平的影响，并确定其作用机制。方法：用表儿茶素处理MCF-7和HT29细胞24小时，并将未处理的细胞作为对照培养物。分离miRNA，并使用qRT-PCR来测量不同致癌和抑癌miRNA的表达谱变化。此外，还筛选了不同浓度表儿茶素的mRNA表达谱。结果：我们的结果显示miRNA的表达水平发生了数倍的变化，这是细胞系特异性的。此外，不同浓度的表儿茶素在两种细胞系中诱导mRNA表达水平的双相变化。结论：我们的研究结果首次表明，表儿茶素可以逆转这些miRNA的表达，并可能在较低浓度下引发细胞抑制作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current molecular pharmacology Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

4.90

自引率

3.70%

发文量

112

期刊介绍： Current Molecular Pharmacology aims to publish the latest developments in cellular and molecular pharmacology with a major emphasis on the mechanism of action of novel drugs under development, innovative pharmacological technologies, cell signaling, transduction pathway analysis, genomics, proteomics, and metabonomics applications to drug action. An additional focus will be the way in which normal biological function is illuminated by knowledge of the action of drugs at the cellular and molecular level. The journal publishes full-length/mini reviews, original research articles and thematic issues on molecular pharmacology. Current Molecular Pharmacology is an essential journal for every scientist who is involved in drug design and discovery, target identification, target validation, preclinical and clinical development of drugs therapeutically useful in human disease.