Oxidative genomic damage in humans exposed to high indoor radon levels in Northeast Brazil

IF 2.3 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2023-07-01 DOI:10.1016/j.mrgentox.2023.503652

Luíza Araújo da Costa Xavier , Julio Alejandro Navoni , Viviane Souza do Amaral

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引用次数: 0

Abstract

Radon gas inhalation is the main source of exposure to ionizing radiation by humans. There is still lack in knowledge concerning the chronic and indirect effects of exposure to this carcinogenic factor. Therefore, the aim of this work is to analyze the levels of oxidative genomic damage in inhabitants of a medium-high background radiation area (HBRA) (N = 82) in Northeastern Brazil and compare them with people living in a low background radiation area (LBRA) (N = 46). 8-hydroxy-2-deoxyguanosine (8-OHdG) was quantified in urine, Ser326Cys polymorphism was determined in the hOGG1 gene and indoor radon was measured. HBRA houses had 6.5 times higher indoor radon levels than those from LBRA (p-value < 0.001). The 8-OHdG mean (95% confidence interval) were significantly different, 8.42 (5.98–11.9) ng/mg creatinine and 29.91 (23.37–38.30) ng/mg creatinine for LBRA and HBRA, respectively. The variables representing lifestyle and environmental and occupational exposures did not have a significant association with oxidized guanosine concentrations. On the other hand, lower 8-OHdG values were observed in subjects that had one mutant allele (326Cys) in the hOGG1 gene than those who had both wild alleles (Ser/Ser (p-value < 0.05). It can be concluded that high radon levels have significantly influenced the genome oxidative metabolism and hOGG1 gene polymorphism would mediate the observed biological response.

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巴西东北部暴露于高室内氡水平的人的氧化基因组损伤

吸入氡气是人类接触电离辐射的主要来源。关于暴露于这种致癌因素的慢性和间接影响的知识仍然缺乏。因此，这项工作的目的是分析巴西东北部中高背景辐射区(HBRA)居民(N = 82)的氧化基因组损伤水平，并将其与生活在低背景辐射区(LBRA)的人(N = 46)进行比较。测定尿中8-羟基-2-脱氧鸟苷(8-OHdG)含量，测定hOGG1基因中Ser326Cys多态性，测定室内氡含量。HBRA住宅的室内氡水平是LBRA住宅的6.5倍(p值<0.001)。LBRA和HBRA的8-OHdG平均值(95%置信区间)差异显著，分别为8.42 (5.98 ~ 11.9)ng/mg肌酐和29.91 (23.37 ~ 38.30)ng/mg肌酐。代表生活方式、环境和职业暴露的变量与氧化鸟苷浓度没有显著关联。另一方面，在hOGG1基因中有一个突变等位基因(326Cys)的受试者的8-OHdG值低于同时有两个野生等位基因的受试者(Ser/Ser (p值<0.05)。由此可见，高氡水平显著影响了基因组的氧化代谢，而hOGG1基因多态性介导了所观察到的生物学反应。

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来源期刊

Mutation research. Genetic toxicology and environmental mutagenesis 生物-毒理学

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

105 days

期刊介绍： Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas: New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results). Alternatives to and refinement of the use of animals in genotoxicity testing. Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials. Studies of epigenetic changes in relation to genotoxic effects. The use of structure-activity relationships in predicting genotoxic effects. The isolation and chemical characterization of novel environmental mutagens. The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures. The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing). MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.