Histologic evaluation of a catheter coated with paclitaxel PLGA nanoparticles in the internal jugular veins of rats.

IF 3.2 4区医学 Q2 ENGINEERING, BIOMEDICAL

Biomedical Engineering Letters Pub Date : 2023-04-29 eCollection Date: 2023-08-01 DOI:10.1007/s13534-023-00282-y

Song-Yi Kim, Susmita Aryal, Wan Su Yun, Woo Cheol Kim, Suk-Bae Moon, Gi-Bong Chae, Jaehong Key, Seongyup Kim

{"title":"Histologic evaluation of a catheter coated with paclitaxel PLGA nanoparticles in the internal jugular veins of rats.","authors":"Song-Yi Kim, Susmita Aryal, Wan Su Yun, Woo Cheol Kim, Suk-Bae Moon, Gi-Bong Chae, Jaehong Key, Seongyup Kim","doi":"10.1007/s13534-023-00282-y","DOIUrl":null,"url":null,"abstract":"The aim of this study is to investigate the potential impact of catheterization on intimal hyperplasia and explore the efficacy of Paclitaxel loaded PLGA nanoparticles (PTX-NPs) in preventing stenosis at the site of venous injury. Under general anesthesia, Central Venous Catheters were inserted into the rat's right internal jugular veins (IJV) using the cut-down technique. Twenty bare catheters (C) and twenty PTX-NPs coated catheters (P) were assigned to one of four groups (C2, C4, P2, or P4) based on catheter type and expected survival time. 2 or 4 weeks after surgery, IJVs were completely harvested by formalin fixation and gelatin infusion and slides were stained with H&E (Haematoxylin and Eosin) and Masson's technique. The P2 (Paclitaxel coating, 2 weeks) group showed the most proliferation among the four groups and the P4 (Paclitaxel coating, 4 weeks) showed a tendency to decrease proliferation. Additionally, the lumen size in the P4 group was about 6% smaller than in the P2 group, and there was a lower prevalence of stenotic grade in the P4 group. Our study suggests that PTX-NPs coated catheters may be effective in preventing venous stenosis if the intended usage is prolonged, rather than for a short-term period.Graphical abstract: Schematic representation of catheter functionalization and coating of PTX-NPs on Catheter.Supplementary information: The online version contains supplementary material available at 10.1007/s13534-023-00282-y.","PeriodicalId":46898,"journal":{"name":"Biomedical Engineering Letters","volume":"13 3","pages":"505-514"},"PeriodicalIF":3.2000,"publicationDate":"2023-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382362/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Engineering Letters","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s13534-023-00282-y","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/8/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}

引用次数: 0

Abstract

The aim of this study is to investigate the potential impact of catheterization on intimal hyperplasia and explore the efficacy of Paclitaxel loaded PLGA nanoparticles (PTX-NPs) in preventing stenosis at the site of venous injury. Under general anesthesia, Central Venous Catheters were inserted into the rat's right internal jugular veins (IJV) using the cut-down technique. Twenty bare catheters (C) and twenty PTX-NPs coated catheters (P) were assigned to one of four groups (C2, C4, P2, or P4) based on catheter type and expected survival time. 2 or 4 weeks after surgery, IJVs were completely harvested by formalin fixation and gelatin infusion and slides were stained with H&E (Haematoxylin and Eosin) and Masson's technique. The P2 (Paclitaxel coating, 2 weeks) group showed the most proliferation among the four groups and the P4 (Paclitaxel coating, 4 weeks) showed a tendency to decrease proliferation. Additionally, the lumen size in the P4 group was about 6% smaller than in the P2 group, and there was a lower prevalence of stenotic grade in the P4 group. Our study suggests that PTX-NPs coated catheters may be effective in preventing venous stenosis if the intended usage is prolonged, rather than for a short-term period.

Graphical abstract: Schematic representation of catheter functionalization and coating of PTX-NPs on Catheter.

Supplementary information: The online version contains supplementary material available at 10.1007/s13534-023-00282-y.

查看原文本刊更多论文

大鼠颈内静脉中紫杉醇PLGA纳米颗粒涂层导管的组织学评价。

本研究的目的是研究导管插入术对内膜增生的潜在影响，并探讨紫杉醇负载的PLGA纳米颗粒（PTX-NPs）在预防静脉损伤部位狭窄方面的疗效。在全身麻醉下，使用切开技术将中心静脉导管插入大鼠右颈内静脉（IJV）。根据导管类型和预期生存时间，将20根裸导管（C）和20根PTX-NP涂层导管（P）分为四组（C2、C4、P2或P4）中的一组。手术后2或4周，通过福尔马林固定和明胶输注完全收获IJVs，并用H&E（苏木精和Eosin）和Masson技术对载玻片进行染色。在四组中，P2（紫杉醇涂层，2周）组显示出最多的增殖，而P4（紫杉醇包衣，4周）显示出减少增殖的趋势。此外，P4组的管腔尺寸比P2组小约6%，P4组狭窄程度的发生率较低。我们的研究表明，如果预期使用时间延长，而不是短期使用，PTX-NP涂层导管可能有效预防静脉狭窄。图形摘要：导管功能化和导管上PTX NP涂层的示意图。补充信息：在线版本包含补充材料，可访问10.1007/s13534-023-00282-y。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biomedical Engineering Letters ENGINEERING, BIOMEDICAL-

CiteScore

6.80

自引率

0.00%

发文量

期刊介绍： Biomedical Engineering Letters (BMEL) aims to present the innovative experimental science and technological development in the biomedical field as well as clinical application of new development. The article must contain original biomedical engineering content, defined as development, theoretical analysis, and evaluation/validation of a new technique. BMEL publishes the following types of papers: original articles, review articles, editorials, and letters to the editor. All the papers are reviewed in single-blind fashion.